| 游碧蓉 陈昶 田新玮 王贵鹏 薛冰 李玲玲.结缔组织病患者活动期血凝增加机制研究[J].,2015,15(27):5312-5315 |
| 结缔组织病患者活动期血凝增加机制研究 |
| Mechanismof Enhanced Blood Coagulation for Connective Tissue DiseasePatients during Active Phase |
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| DOI： |
| 中文关键词: 结缔组织病 嗜酸性粒细胞 组织因子 血液凝固 |
| 英文关键词: Connective tissue disease Eosinophil Tissue factor Blood coagulation |
| 基金项目:新疆自治区科技计划项目（201391348） |
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| 中文摘要: |
| 目的：评价不同结缔组织病患者活动期和健康对照者血液中嗜酸性粒细胞中组织因子(TF)表达的变化。方法：研究对象为9
例处于活动期的不同类型结缔组织病患者和9 例健康对照者，分离结缔组织病患者和正常对照者外周血中嗜酸性粒细胞。用
western blot和RT-PCR检测嗜酸性粒细胞中TF蛋白及mRNA水平变化情况。结果：发现结缔组织病患者嗜酸性粒细胞中TF蛋
白表达水平显著高于正常对照者(P<0.001)；结缔组织病患者嗜酸性粒细胞中TF 的循环阈值(Ct)中位数是35.10 (19.45-36.50)，正
常对照者为37.17 (35.33-37.87)，患者中TF mRNA水平明显高于正常对照者(p = 0.002)。结论：我们的结果证实人血液嗜酸性粒细
胞中TF表达会发生变化。结缔组织病患者活动期高表达TF可能有助于血液凝固性增加。 |
| 英文摘要: |
| Objective:To investigate whether tissue factor (TF) expression varies in eosinophils isolated from normal subjects and
patients with connective tissue disease.Methods:Eosinophils were immunologically purified from peripheral blood samples of 9 patients
with different connective tissue disease and 9 normal subjects. Western blot analysis and real-time polymerase chain reaction (RT-PCR)
were performed to test eosinophil TF expression.Results:Western blot analysis revealed TF in homogenates of purified eosinophils with
a higher intensity in the 9 patients than in the 9 controls (P<0.001). According to RT-PCR cycle threshold (Ct), TF gene expression was
higher in eosinophils from patients than in those from controls, median (range) 35.10 (19.45-36.50) vs 37.17 (35.33-37.87) (P = 0.002).Conclusion:Our results indicate that TF expression may vary in human blood eosinophils. The higher TF expression in patients with connective
tissue disease may contribute to increasing the blood coagulation. |
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