| 许鹏 王瑞峰 孟宪红 周婷 韩继武.非酒精性脂肪性肝病与MTP关系的实验研究[J].,2015,15(27):5234-5237 |
| 非酒精性脂肪性肝病与MTP关系的实验研究 |
| Study on the Relationship of MTP with Non-alcoholic Fatty Liver Disease |
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| DOI： |
| 中文关键词: 非酒精性脂肪性肝病 微粒体甘油三酯转运蛋白 抑制剂 |
| 英文关键词: Non-alcoholic fatty liver disease Microsomal triglyceride transfer protein Inhibitor |
| 基金项目:黑龙江省普通高等学校医学遗传学重点实验室开放课题资助(20101324); 哈尔滨市科技创新人才研究专项资金(2014RFXGJ043) |
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| 中文摘要: |
| 目的：探讨非酒精性脂肪性肝病( Non-alcoholic fatty liver disease, NAFLD)与微粒体甘油三酯转运蛋白(microsomal triglyceridetransfer
protein，MTP）的关系。方法：将雄性Wistar 大鼠60只随机分为正常对照组（A 组）、高脂组（B 组）和MTP 抑制剂组（C
组），每组各20 只。B 组、C组给予高脂饲料喂养，8 周后确认非酒精性脂肪肝建模成功，C组大鼠给予混有特异性小肠MTP抑制
剂JTT-130 的高脂饲料喂养，B 组大鼠建模过程始终喂养高脂饲料，A 组大鼠喂养普通饲料。于第12周，分别测定大鼠血清甘油
三酯(triglyceride，TG)、总胆固醇(total cholesterol，TC)、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol，HDL-c)含量，以及
肝脏TC、TG、磷脂含量。同时测定肝脏中微粒体甘油三酯转运蛋白(MTP) 的活性与mRNA 表达量。结果：与正常对照组（A组）相
比，高脂组（B组）大鼠血清TC、TG、HDL-c 浓度和肝脏TC、TG含量明显提高（P＜0.05），MTP 活性及mRNA 水平明显下调（P＜
0.05）。与高脂组（B 组）比较，MTP 抑制剂组（C 组）大鼠血清TC、TG、HDL-c 浓度和肝脏TC、TG 含量明显下降（P＜0.05），而
MTP 活性及mRNA 表达量比较无明显差别（P＞0.05）。结论：非酒精性脂肪性肝病存在MTP表达下调，特异性小肠MTP 抑制剂
JTT-130 可以有效抑制肠道对TG的转运，不影响肝脏TG分泌，并在降低高脂大鼠血浆TG和胆固醇水平的同时也降低肝脏TG
含量。 |
| 英文摘要: |
| Objective:To explore the relationship between Non-alcoholic fatty liver disease (NAFLD) and microsomal triglyceride
transfer protein (MTP).Methods:60 male Wistar rats were randomly divided into normal control group (group A), high fat group (group
B), and MTP inhibitors group (group C). Group B and group C were given high fat feed. 8 weeks after successful establishment of the
model of nonalcoholic fatty liver disease, the rats in group C was given high fat diet mixed with specificity of intestinal MTP inhibitor
JTT-130, and the rats in high-fat group was fed high-fat feed all the time, while the rats of the control group was fed regular feed. After
12 weeks, the levels of TG, TC, HDL-c in rat serum and TC, TG, phospholipid in the intestinal content were measured. And the activity
and the mRNA expression of liver MTP was determined.Results:Compared with the normal control group, the levels of TG, TC, HDL-c
in rat serum and TC, TG, phospholipid in the intestinal content in group B were higher than those in normal control group, and the
difference was statistically significant (P<0.05). The activity and mRNA levels of MTP were significantly decreased (P<0.05). Compared
with the high fat diet group (group B), the concentration of serum TC, TG, HDL-c and the TC, TG content in liver was decreased
significantly in the rats of MTP inhibitor group (group C) (P<0.05), and no significant differences of MTP activity and mRNA expression
(P>0.05).Conclusion:MTP expression is downregulated in non-alcoholic fatty liver disease, while the specific intestinal MTP inhibitor
JTT-130 can effectively inhibit the intestinal transport of TG, but not affect the hepatic TG secretion, and it can also decrease plasma TG
and cholesterol levels and TG content in liver of high fat rats. |
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