| 丁丽玉 王浣清 吴飞 刘春林 林安琪 章悦蕾 程艳梅 朱生樑.高效液相色谱法测定藿苏养胃口服液中黄芪甲苷的含量[J].,2015,15(27):5216-5220 |
| 高效液相色谱法测定藿苏养胃口服液中黄芪甲苷的含量 |
| Determination of Astragaloside IV in Huosuyangwei Oral Liquid |
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| DOI： |
| 中文关键词: 藿苏养胃口服液 黄芪甲苷 高效液相色谱法 前处理 |
| 英文关键词: Huosuyangwei Oral Liquid Astragaloside IV HPLC Pretreatment |
| 基金项目:上海市科委科研基金项目(11DZ1972002) |
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| 中文摘要: |
| 目的：建立藿苏养胃口服液中黄芪甲苷前处理及含量的HPLC 测定方法。方法：前处理方法如下：100 mL样品中加入67 mL
浓氨水，振荡后静置5 min，转移至D101大孔树脂柱上，上样完毕后，依次以100 mL 氨试液、100 mL 水和100 mL 40%乙醇洗脱，
弃去洗脱液，然后以100 mL 70%乙醇洗脱，收集洗脱液，蒸干，用甲醇溶解并转移至5 mL 容量瓶中，以甲醇溶解；测定方法如下：
采用Agilent ZORBAX Eclipse XDB C18（4.6× 150 mm，5.0 滋m）色谱柱，以乙腈- 水（33:67）为流动相，流速1.0 mL·min-1，柱温35
℃；ELSD 检测器的参数为：氮气流速1.60 SLM、雾化器温度30 ℃、蒸发器温度80 ℃。结果：黄芪甲苷线性范围为0.262~15.696
滋g（r = 0.9992），平均加样回收率为99.46%（n=6），RSD为2.3%。结论：所建立的前处理方法简便易行，准确度高，重复性好；检测
方法可作为藿苏养胃口服液的质量控制方法。 |
| 英文摘要: |
| Objective:To establish a HPLC method for the pretreatment and determination of Astragaloside IV in Huosuyangwei
Oral Liquid.Methods:The pretreatment method was as follows: 67 mL ammonium hydroxide was added into 100 mL sample, shaked
and stood for 5 mins, then transfered to the column filled with D101 macroporous resin. And then to elute it with 100 mL of water, 100
mL of 40%ethanol orderly and discard the elution buffer. Finally, 100 mL 70%ethanol was added into the column to collect the sample,
the elution buffer was gathered and evaporated to dryness, which was dissolved with methanol and transfered into 5 mL volumetric flask.
The HPLC-ELSD determination method was as follows: the separation was performed on an Agilent ZORBAX Eclipse XDB C18 analytical
column (4.6× 150 mm, 5.0 滋m I.D) with mobile phase consisting of acetonitrile-water (33: 67). The flow rate was 1.0 mL·min-1,
while the column was kept at 35 ℃ . ELSD was employed as the detector. The temperature of nebuliser and evaporator was set at 30℃
and 80℃, respectively. The flow rate of N2 was 1.60 SLM.Results:The linear of Astragaloside IV was 0.262-15.696 滋g (r = 0.9992) and
the average recovery was 99.46%with RSD of 2.3% (n=6).Conclusion:The pretreatment method with a high accuracy was simple and
convenient. The HPLC method of Astragaloside IV determination was suitable for the quality control of Huosuyangwei Oral Liquid. |
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