| 屈小玲 李晨 张旭涛 孟瑾 张涛.转录因子WSTF对肺癌细胞增殖和侵袭的实验研究[J].,2015,15(25):4848-4851 |
| 转录因子WSTF对肺癌细胞增殖和侵袭的实验研究 |
| WSTF Promotes Proliferation and Invasion of Lung Cancer Cells |
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| DOI： |
| 中文关键词: 肺癌细胞 WSTF 增殖 迁移 侵袭 |
| 英文关键词: Lung cancer cells WSTF Proliferation Migration Invasion |
| 基金项目:国家自然科学基金面上项目（81172223） |
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| 中文摘要: |
| 目的：研究转录因子WSTF 对肺癌细胞增殖和侵袭作用的影响。方法：采用慢病毒介导的基因转染方法建立A549 细胞
WSTF 高表达细胞系A549-WSTF 和空质粒对照细胞系A549-control。细胞增殖实验和克隆形成实验观察ING5 过表达对肺癌
A549 细胞增殖能力的影响；Trans-well 迁移实验和侵袭实验观察WSTF对肺癌细胞迁移和侵袭能力的影响。结果：Western blot验
证A549-WSTF 细胞WSTF蛋白水平显著高于对照细胞A549-control，P=0.0004。WSTF 高表达明显促进了肺癌细胞的增殖能力
（1-4 天P 值分别为0.002、0.0004、0.0002 和3.21× 10-5）和克隆形成能力（P=0.004）；WSTF 过表达还显著促进了肺癌细胞从
trans-well 小室迁移到下室的作用，其OD570 值分别为0.626± 0.013（A549-WSTF）和0.322± 0.010（A549-control），P=2.37× 10-5；
WSTF还促进肺癌细胞穿透基质胶迁移到下室，其OD570 值分别为0.600± 0.027（A549-WSTF）和0.333± 0.017（A549-control），
P=0.0004。结论：WSTF可以促进肺癌细胞的增殖和侵袭能力而发挥促癌作用。 |
| 英文摘要: |
| Objective:To investigate the effects of WSTF on the proliferative and invasive abilities of lung cancer cells.Methods:WSTF stably overexpressed A549 cell line (A549-WSTF) and control A549 cell line (A549-control) were established by lentiviral
infection. Proliferation assay and colony formation assay were used to observe the effects of WSTF overexpression on the proliferative
ability of lung cancer cells. Trans-well migration assay and invasion assay were performed to study the influence of WSTF on invasive
capacity of lung cancer cells.Results:WSTF protein level in A549-WSTF cells was significantly higher than that of control cells (P=0.
0004). WSTF overexpression significantly promotes proliferation (P=0.002, 0.0004, 0.0002 and 3.21× 10-5 on day 1 to day 4) and colony
formation abilities (P=0.004) of lung cancer A549 cells. In addition, WSTF overexpression increases the migration ability of lung cancer
cells indicated by migrated cell OD570 value, with A549-WSTF (0.626± 0.013) much higher than A549-control (0.322± 0.010) (P=2.
37× 10-5). Similar results were observed in trans-well invasion assay with the OD570 value of A549-WSTF (0.600± 0.027) much higher
than that of A549-control (0.333± 0.017) (P=0.0004).Conclusion:WSTF may act as an oncoprotein by promoting the proliferative and
invasive abilities of lung cancer A549 cells, and thus could be a promising therapeutic target for the clinical treatment of lung cancer. |
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