| 吴小光 陈敏 李正钰 郭韧.EPHX2基因多态性与长沙地区汉族人群脑梗塞继发性癫痫遗传易感性的关联研[J].,2015,15(25):4805-4808 |
| EPHX2基因多态性与长沙地区汉族人群脑梗塞继发性癫痫遗传易感性的关联研 |
| Study of EPHX2 Polymorphismand Post-stroke Epilepsy Susceptibility inChangsha Han Population |
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| DOI： |
| 中文关键词: 脑梗塞 继发性癫痫 多态性 |
| 英文关键词: EPHX2 Cerebral infarction Secondary epilepsy Polymorphism |
| 基金项目:国家自然科学基金项目（81000120） |
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| 摘要点击次数: 538 |
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| 中文摘要: |
| 目的：研究EPHX2rs1042032 (A/G)多态性与长沙地区汉族人群脑梗塞继发性癫痫遗传易感性关联关系。方法：收集中南大
学湘雅医院及湘雅三医院脑梗塞未继发癫痫及脑梗塞继发癫痫患者血液标本。利用病例-对照研究方法，将标本分为对照组（即
无EP 组）402 例和病例组（即EP组）124 例，提取血样的DNA。然后根据人类基因组DNA序列，设计EPHX2rs1042032 PCR引
物，用PCR 方法对目的基因进行扩增。通过聚合酶链反应- 限制性片段长度多态性（PCR- restriction fragment length
polymorphism，PCR-RFLP）方法，先对扩增的目的基因片段进行酶切，然后通过凝胶电泳对不同的等位基因进行分辨。结果：EP组
和无EP组间EPHX2rs1042032 多态位点基因型分布无显著统计学差异（P>0.05）。结论： EPHX2rs1042032 的基因多态性与脑梗
塞继发性癫痫可能无关。 |
| 英文摘要: |
| Objective:To explore EPHX2 polymorphism and post-stroke epilepsy susceptibility in Changsha Han population.Methods:Using a case-control study, we collected the blood samples from 402 stroke patients without epilepsy and 124 post-stroke
epilepsy patients in Xiangya Hospital and The Third Xiangya Hospital of Central South University. The subjects were divided into case
group (EP group) and the control group (non EP group), genomic DNA were extracted from blood samples. Then, we designed EPHX2
rs1042032 PCR primers according to the DNA sequence of the human genome, the target gene was amplified by PCR. By
polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method, the PCR products were digested by restriction endonuclease Ⅰ, restricted DNA products were then separated by using 3%agarose gel electrophoresis and visuslized by UV light.Results:The frequency distribution of EPHX2rs1042032 between EP group and non EP group was not significantly different.Conclusion:The data suggests that EPHX2rs1042032 may not be associated with post-stroke epilepsy. |
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