| 李晓明 杨莹 彭辉 刘永莹 龙钦鹏.FXR 在糖尿病大鼠回肠的表达水平及意义[J].,2015,15(24):4640-4644 |
| FXR 在糖尿病大鼠回肠的表达水平及意义 |
| FXR Expression Level in Ileumof Diabetic Rats Model and Its Significance |
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| DOI： |
| 中文关键词: FXR 糖尿病 回肠 STZ |
| 英文关键词: FXR Diabetes Ileum STZ |
| 基金项目:国家自然科学基金项目(81260135) |
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| 中文摘要: |
| 目的：探索法尼酯衍生物受体(farnesoid X receptor, FXR)在链脲佐菌素(Streptozotocin，STZ)诱导的1 型糖尿病(diabetes mellitus，
DM)大鼠模型回肠组织中的表达水平及意义。方法：将大鼠随机分为正常对照组(NC 组)和DM模型组(DM组)。腹腔注射
STZ建立DM 大鼠模型，NC 组予无水乙醇生理盐水溶液腹腔注射。通过实时荧光定量PCR检测FXR在回肠组织mRNA表达水
平。结果：(1)DM 组FXR mRNA 表达量减少、糖化血红蛋白升高、平均血糖升高、体重下降与NC组之间的差异有统计意义(P<0.
05)；(2) FXR mRNA表达量pearson相关分析：DM组平均血糖、糖化血红蛋白、最终体重与最高体重相比下降的程度、最终体重与
造模时体重相比下降的程度与FXR mRNA表达量呈负相关；（3) FXR mRNA表达量多元线性回归分析：DM组平均血糖、最终体
重与最高体重相比下降的程度、糖化血红蛋白对FXR mRNA表达量的影响大(P<0.05)，而NC 组各个指标对FXR mRNA 表达量
无影响(P>0.05)。结论：FXR表达异常与糖尿病的发病和糖尿病患者的体重下降相关。 |
| 英文摘要: |
| Objective:To explore the FXR expression level in the ileum of diabetic rats induced by Streptozotocin and its clinical
significance.Methods:The rats were randomly divided into normal control group (NC group) and diabetes mellitus model group (DM
group) , respectively. The diabetic rats model was established by STZ intraperitoneal injection. By real-time fluorescent quantitative Polymerase
Chain Reaction, FXR mRNA expression level was detected in the tissue of ileum.Results:①The difference between DMgroup
and NC groups in FXR mRNA expressionreducing, glycosylated hemoglobin rising, the average blood glucose lifting, body weight losing
had statistical significance (P<0.05); ②Pearson correlation analysis showed the level of FXR mRNA expression was negatively correlated
with the glycosylated hemoglobin, average blood glucose, the degree of weight loss compared to the final weight and the highest
weight, the difference between the final weight and the weight in the establishment of model in DMgroup (P<0.05); ③multiple linear regression
analysis showed the level of FXR mRNA expression was affected by the glycosylated hemoglobin, average blood glucose, the
degree of weight loss compared to the final weight and the highest weight, in DMgroup (P<0.05), but the indicators in NC group did not
impact the amount of FXR mRNA expression level(P>0.05).Conclusion:Abnormal FXR expression is associated with the onset of diabetes
and the weight loss of diabetes patients. |
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