| 谌海燕 王云涛 韩福旺 丁晓庆 郭明 陈信义.复方浙贝颗粒联合三苯氧胺对LCC2 移植瘤相关蛋白表达影响[J].,2015,15(24):4610-4615 |
| 复方浙贝颗粒联合三苯氧胺对LCC2 移植瘤相关蛋白表达影响 |
| Effect of Compound Zhe Bei Granul Combined with Tamoxifen on theExpression of Associated Protein of LCC2 Xenografts |
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| DOI： |
| 中文关键词: 复方浙贝颗粒 三苯氧胺 乳腺癌 移植瘤 |
| 英文关键词: Compound Zhe Bei granul Tamoxifen Breast cancer Xenografts |
| 基金项目:北京中医药大学自主创新课题(2009TYBZZ-XS057) |
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| 中文摘要: |
| 目的：研究复方浙贝颗粒(CZBG)联合三苯氧胺(TAM)对LCC2移植瘤组织相关蛋白HER-2、GST-π、Caspase3 表达的影响。
方法：用耐TAM的人乳腺癌细胞系LCC2 建立小鼠移植瘤模型。实验分模型对照组，TAM 组，阿霉素(ADM)+TAM 组，中剂量
CZBG+TAM组，ADM+中剂量CZBG+TAM组，采用免疫组化法分别检测各组HER-2、GST-π、Caspase3 的表达差异。结果：①与
TAM 组相比，中剂量CZBG+TAM组、ADM+ 中剂量CZBG+TAM组能下调HER-2 表达(P<0.05)。②与模型对照组相比，各治疗
组GST-π表达均有所下降(P<0.05)；各治疗组之间的GST-π表达无统计学意义(P>0.05)。③与模型对照组相比，中剂量
CZBG+TAM组和ADM+中剂量CZBG+TAM 组均能上调Caspase3 表达(P<0.05)；中剂量CZBG+TAM 组与模型对照组、TAM
组、ADM+TAM组比较，Caspase3 的表达均有统计学意义(P<0.05)。结论：中剂量CZBG 能够下调乳腺癌LCC2 细胞移植瘤组织的
HER-2 表达，提高Caspase3 活性，而对GST-π表达无明显影响。 |
| 英文摘要: |
| Objective:To study the expression of associated protein HER-2，GST-π，Caspase3 of LCC2 xenografts treated by
Compound Zhe Bei granul(CZBG) combined with Tamoxifen(TAM).Methods:Xenograft model was made by using human breast cancer
cells LCC2 resistant to TAM. Then the mice were randomly divided into model control group, simple TAMgroup, adriamycin(ADM)
+TAM group, middle dose of CZBG+TAM group and ADM+middle dose of CZBG+TAM group. Differential expression of HER-2,
GST-π, Caspase3 of each group was detected by using immunohistochemistry, respectively.Results:① Middle dose of CZBG+TAM
group and ADM+middle dose of CZBG+TAM group could down-regulate the expression of HER-2 compared with simple TAM group,
which had statistical differences (P<0.05). ② The expression of GST-π in each treatment group declined compared with model control
group (P<0.05). The expression of GST-π between treatment groups had no significant differences (P>0.05). ③The expression of Caspase3
in middle dose of CZBG+TAM group and ADM+middle dose of CZBG+TAM group was increased significantly compared with
model control group, which had statistical differences(P<0.05). And the expression of Caspase3 in middle dose of CZBG+TAMgroup also
had statistical differences compared with simple TAM group and ADM+TAM group (P<0.05).Conclusion:Middle dose of CZBG
can down-regulate HER-2 expression of LCC2 xenografts, improve Caspase3 activity, but has no effect on GST-π expression. |
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