| 杨俊荣 张京慧 王立坤 张所宏 赵红燕 高广慧 徐文鑫 王秋实 徐广全.缺氧对食管癌细胞增殖及凋亡的影响[J].,2015,15(23):4457-4461 |
| 缺氧对食管癌细胞增殖及凋亡的影响 |
| The Influence of Hypoxia on Proliferation and Apoptosis ofEca109 Esophageal Carcinoma Cells |
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| DOI： |
| 中文关键词: 食管癌 Eca109 缺氧 增殖 凋亡 |
| 英文关键词: Esophageal carcinoma Eca109 Hypoxia Proliferation Apoptosis |
| 基金项目:黑龙江省卫生厅科研课题(2013277) |
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| 中文摘要: |
| 目的：探讨缺氧对人食管癌细胞Eca109 增殖及凋亡的影响及其作用机制，为食管癌的诊断和治疗提供新的思路和路径。方
法：以终浓度为250 umol/L 氯化钴模拟缺氧环境，将人食管癌细胞Eca109 于常氧及缺氧条件下分别培养12 h、24 h、36 h、48 h，
采用倒置相差显微镜观察细胞生长情况，MTT 法检测细胞增殖情况，利用细胞活力分析仪NC-3000 检测各组细胞凋亡情况。结
果：相对于常氧对照组而言，缺氧后各组Eca109细胞增殖减弱，凋亡细胞比率明显升高，其中以缺氧24 h的凋亡细胞比率最高，
缺氧36 h的凋亡细胞比率较缺氧24 h时有所回落。结论：缺氧可以抑制人食管癌细胞Eca109 的增殖并诱导其凋亡。 |
| 英文摘要: |
| Objective:Investigate the influence of hypoxia on proliferation and apoptosis of Eca109 cells and their mechanisms to
provide new ideas and pathways for the diagnosis and treatment of esophageal cancer.Methods:The hypoxia is induced by incubation of
Eca109 cells with cobalt chloride at 250 umol/L. Eca109 cells are respectively cultured in normoxia and hypoxia after 12 h, 24 h, 36 h
and 48 h. The morphologic changes, growth and proliferation are observed by inverted microscope. The proliferation is detected by MTT.
Taking advantage of Nucleocounter NC-3000 to analyze the cell apoptosis of each group.Results:The proliferation abilities of Eca109
cells cultured in hypoxia are weakened, but the apoptotic ratio of cells is higher than that in the normoxia group. Among them, the cells
cultured in hypoxia after 24 h have the highest apoptotic ratio. And then the apoptotic ratio of Eca109 cells cultured in hypoxia after 36h
falls back a little compared with the cells cultured in hypoxia after 24 h.Conclusion:Hypoxia can inhibit the proliferation and induce the
apoptosis of human esophageal carcinoma cells. |
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