| 尹小菲 谭晓华 李靖 何敏 杨磊.vFLIP激活Jurkat细胞表达HIV 抑制因子的研究[J].,2015,15(23):4449-4452 |
| vFLIP激活Jurkat细胞表达HIV 抑制因子的研究 |
| vFLIP Stimulates Anti-HIV Factors Expression in Jurkat Cells |
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| DOI： |
| 中文关键词: KSHV HIV抑制因子 vFLIP Jurkat |
| 英文关键词: KSHV HIV Inhibitory Factors vFLIP Jurkat |
| 基金项目:浙江省科技厅国际合作重点项目（2009C14014） |
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| 中文摘要: |
| 目的：检测KSHV 编码的vFLIP 蛋白对Jurkat 细胞HIV 抑制因子表达的影响。方法：本研究首先构建真核表达载体
pIRES2-EGFP-vFLIP，再将其电穿孔至Jurkat 细胞，然后使用实时荧光定量PCR 技术检测Jurkat 细胞中A3G、A3F、CCL5 等HIV
抑制因子的表达水平，从而探讨vFLIP 抗HIV/AIDS 的作用及机制。结果：成功构建了真核表达载体pIRES2-EGFP-vFLIP，电转
染效率达到40%左右。与对照载体转染组相比，vFLIP转染组内Jurkat 细胞中CCL5、A3G、A3F 及Mx2 基因的表达分别上调了
6.71、2.20、1.52 及14.47 倍。结论：vFLIP 蛋白可以激活Jurkat细胞内某些HIV 抑制因子的表达，提示其具有一定的抗HIV/AIDS
作用。 |
| 英文摘要: |
| Objective:To detect the effect of vFLIP on expression levels of anti-HIV factors in Jurkat cells.Methods:In this study,
we constructed the eukaryotic expressive vector of pIRES2-EGFP-vFLIP and transfected the vector to Jurkat cells by electroporation.
Then we detected the relative expression levels of anti-HIV factors in vFLIP transfected Jurkat cells by qPCR. Finally, we discussed the
functions and mechanisms of vFLIP against HIV/AIDS.Results:The eukaryotic expressive vector of pIRES2-EGFP-vFLIP was
successfully constructed. The transfection efficiency of vectors were about 40%. In comparison with pIRES2-EGFP transfected cells, the
pIRES2-EGFP-vFLIP transfected cells can increase expression levels of CCL5, A3G, A3F and Mx2 in Jurkat cells by 6.71, 2.20, 1.52
and 14.47 times seperately.Conclusion:vFLIP can activate expressions of CCL5, A3G, A3F and Mx2 in Jurkat cells, and thus suggesting
that vFLIP may have the function of anti-HIV/AIDS . |
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