| 丁静云 应颖 邬磊 伍茵 孔祥臣 曾庆 梁真.INS-1E 细胞葡萄糖毒性模型的建立[J].,2015,15(22):4244-4247 |
| INS-1E 细胞葡萄糖毒性模型的建立 |
| Establish Glucose ToxicityModel of INS-1E Cell |
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| DOI： |
| 中文关键词: INS-1E 细胞 葡萄糖毒性 胰岛素 |
| 英文关键词: INS-1E cell Glucose toxicity Insulin |
| 基金项目:深圳市科技计划重点项目（201101006）；深圳市科技研发资金基础研究项目（JC201105180813A） |
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| 中文摘要: |
| 目的：建立胰岛细胞系INS-1E细胞的葡萄糖毒性模型。方法：将INS-1E 细胞分别在不同葡萄糖浓度（5.5 mmol/L、16.7
mmol/L、25 mmol/L、30 mmol/L）的1640 完全培养基中培养不同时间（48 h、72 h、96 h、120 h），分别在不同时间点取细胞进行细胞
功能检测，实时荧光定量PCR法检测胰岛素mRNA 的表达，ELISA 检测葡萄糖刺激的胰岛素的分泌。结果：与对照组相比，高糖
浓度（5.5 mmol/L、16.7 mmol/L、25 mmol/L、30 mmol/L）培养基中培养48 h后，INS-1E细胞的胰岛素合成和分泌的功能均增加（P
均<0.05），随着培养基中葡萄糖浓度的升高以及培养时间的延长，INS-1E 细胞胰岛素合成及分泌的功能逐渐下降，当在葡萄糖浓
度为30 mmol/L的培养基中培养120 h后，胰岛素mRNA 合成及葡萄糖刺激的胰岛素分泌均显著降低（P 均<0.01）。结论：INS-1E
细胞在30 mM的葡萄糖中培养120 h形成稳定的葡萄糖毒性模型。 |
| 英文摘要: |
| Objective:To establish glucose toxicity model of INS-1E cell.Methods:INS-1E cells were cultured in different
glucose concentration (5.5 mmol/L, 16.7 mmol/L, 25 mmol/L and 30 mmol/L) 1640 fully culture medium in different time (48 h, 72 h, 96
h, 120 h). Cells were taken at different time points to detect cell function. Insulin mRNA was detected by fluorescent quantitative PCR.
Insulin secretion was tested by ELISA.Results:Compared with the normal glucose control group, after 48 h cultured in high glucose
concentrations (5.5 mmol/L, 16.7 mmol / L, 25 mmol / L, 30 mmol / L), insulin synthesis and secretion of INS-1E cells were increased
(P<0.05). With increasing concentration of glucose in the medium and longer incubation time, insulin synthesis and secretion function of
INS-1E cells was gradually declined. When cells were cultured 120 h in the medium with 30 mmol/L glucose concentration, insulin
mRNA synthesis and glucose-stimulated insulin secretion were significantly reduced (P<0.01).Conclusion:Glucose toxicity model can
be formed after INS-1E cell cultured 120 h in 30 mMglucose. |
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