| 马晓磊 吕安林 姜晓宇 邱翠婷 李珊 郭显.AIBP基因表达下调促进心肌微血管内皮细胞血管新生[J].,2015,15(22):4230-4234 |
| AIBP基因表达下调促进心肌微血管内皮细胞血管新生 |
| Knockdown AIBP Promotes Angiogenesis of Cardiac MicrovascularEndothelial Cells |
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| DOI： |
| 中文关键词: AIBP 微血管内皮细胞 血管新生 |
| 英文关键词: P Microvascular endothelial cells Angiogenesis |
| 基金项目:国家自然科学基金项目（81270263） |
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| 中文摘要: |
| 目的：探讨阻断载脂蛋白A-I结合蛋白（AIBP）的表达后对心肌微血管内皮细胞（CMECs）血管新生的作用。方法：消化法分
离SD 大鼠CMECs，通过慢病毒介导的siRNA转染CMECs 下调AIBP基因表达，并设立空白对照组及阴性转染组。RT-PCR法
检测AIBP基因的表达；CCK-8 比色法检测细胞增殖；Transwell 小室评价细胞迁移能力；成管实验评价血管新生能力。结果：
RT-PCR结果显示，与空白对照组及阴性转染组相比，转染组CMECs 中AIBP表达显著降低（P<0.01）；CCK-8 结果显示，与空白
对照组及阴性转染组相比，转染组CMECs增值水平显著增高（P<0.01）；Transwell 法结果显示，与空白对照组及阴性转染组相比，
转染组CMECs 迁移能力显著增高（P<0.01）；成管实验显示，与空白对照组及阴性转染组相比，转染组CMECs 成管能力显著增高
（P<0.01）。结论：抑制AIBP表达可以明显促进CMECs增殖，促进其迁移和成管。 |
| 英文摘要: |
| Objective:To explore the influence of knockdown the high expressed gene AIBP by RNA interference on
angiogenesis in cardiac microvascular endothelial cells (CMEC).Methods:CMECs was isolated from rats with collagenase digestion, the
transfected group, blank control group and negative control group were included. The transfected efficiency was detected by RT-PCR.
The effect of AIBP-knockdown on angiogenesis on cell proliferation was analyzed by CCK-8 assay, cell migration was assessed by using
migration assays and cell's angiogenic activity was studied by tube formation experiment.Results:The lentivirus vector infected the
CMECs steadily and efficiently. Compared with the control groups, the expression of AIBP of transfected group was significantly
decreased (P<0.01). The cell proliferation of transfected group was enhanced (P<0.01), cell migration and angiogenesis of transfected
group was promoted (P<0.01).Conclusion:Knockdown the high expressed gene AIBP can significantly enhance CMECs proliferation,
promote CMECs migration and angiogenesis. |
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