| 王莉 汪廷乐 孟冉 唐天鸿 宋萌 潘劲松.病理性周期性张应力诱导人牙周膜细胞凋亡的机制研究[J].,2015,15(22):4210-4213 |
| 病理性周期性张应力诱导人牙周膜细胞凋亡的机制研究 |
| Mechanismof Pathology Cyclic Strain-inducedApoptosis in Human Periodontal Ligament Cells |
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| DOI： |
| 中文关键词: 细胞凋亡 人牙周膜细胞 病理性周期性张应力 caspase-3 PARP |
| 英文关键词: Apoptosis Human periodontal ligament cells Pathology cyclic strain Caspase-3 PARP |
| 基金项目:国家自然科学基金项目（11172177）；上海交通大学医工理交叉基金项目（YG2010MS07） |
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| 中文摘要: |
| 目的：阐明病理性周期性张应力诱导人牙周膜细胞凋亡的分子机制。方法：人牙周膜细胞取自健康前磨牙，经过3?5 代传代，
细胞受到20 %牵张力，时间为6 h或24 h，通过用膜联蛋白异硫氰酸荧光素（V-FITC）和碘化丙啶（PI）结合流式细胞仪检测细胞
凋亡，用Western Blot 法研究caspase-3，cleaved caspase-3，116 kDa PARP-1 和85 kDa PARP-1 蛋白的表达变化。结果：人PDL 细
胞受到病理性周期性张应力时存在凋亡，并以一种时间依赖的方式增加。受到病理性周期性张应力后裂解的caspase-3 和PARP
蛋白随着时间增加，然而抑制caspase-3 的活性却可以抑制细胞的凋亡，但并不能抑制由其他通路导致的凋亡。结论：病理性周期
性张应力通过caspase-3/PARP 途径诱导人牙周膜细胞的凋亡。 |
| 英文摘要: |
| Objective:To elucidate the molecular mechanisms by which pathology cyclic strain induces the apoptosis of human
periodontal ligament (PDL) cells.Methods:The human PDL cells were obtained from healthy premolars. After three to five passages, the
cells were stretched by 20 % strain for 6 h and 24 h, then the apoptosis were detected through annexin fluorescein isothiocyanate
(V-FITC) and propidium iodide (PI) binding by flow cytometry, and the protein expression of caspase-3 and PARP was investigated by
Western Blot.Results:Human PDL apoptotic cells were increased in a time-dependent manner in response to pathology cyclic strain.
Cleaved caspase-3 and PARP protein was increased by pathology cyclic strain along with time. Furthermore, inhibition of caspase-3
prevented stretch-induced apoptosis but did not prevent all apoptosis caused by other signaling pathways.Conclusion:Our results
suggested that pathology cyclic strain induced apoptosis in human PDL cells through caspase-3/PARP pathway. Our findings may
provide a novel insight into the mechanismof apoptosis induced by pathology cyclic strain in human PDL cells. |
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