| 李东旭 乔友备 吴红 周健.正交试验法优选PP1 脂质体的制备工艺[J].,2015,15(20):3822-3825 |
| 正交试验法优选PP1 脂质体的制备工艺 |
| Study on Preparation of PP1 Liposome |
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| DOI： |
| 中文关键词: PP1 脂质体 薄膜水化法 |
| 英文关键词: PP1 Liposome Thin filmhydration technique |
| 基金项目:陕西省科技统筹创新工程项目（2012KTCQ03-03）；国家自然科学基金项目（81370998） |
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| 中文摘要: |
| 目的：制备PP1 脂质体，筛选最优处方。方法：用薄膜水化法制备PP1 脂质体，以高效液相色谱法（HPLC）测定PP1 脂质体的
包封率，以包封率为主要指标，选取药脂比、胆固醇磷脂比、温度和水化时间为因素，用正交试验筛选最优配方。结果：用薄膜水法
制备的PP1 脂质体的最优处方的组成为：药脂比为1:10，胆固醇与二棕榈酰磷脂酰胆碱（DPPC）比为1:8，温度为40 ℃，水化时间
为3 min。平均包封率为（63.27± 3.32）%。PP1 脂质体的平均粒径为（157.73± 9.74）nm，Zeta 电位为（-4.74± 0.44）mV。结论：用薄
膜水化法制备出的PP1 脂质体包封率高，形态和粒径均匀，重现性好，为研究其在眼部的缓释作用奠定了基础。 |
| 英文摘要: |
| Objective:To prepare PP1 liposome and screen the optimal formula.Methods:PP1 liposome was prepared by the thin
film hydration technique and the encapsulation efficiency was tested by HPLC. The encapsulation efficiency was the index, and the
optimal formula of the preparation was screened using the orthogonal experiment which was designed with four factors of drug to lecithin
ratio, cholesterol to lecithin ratio, temperature, and sonication time.Results:The optimal formula of the PP1 liposome was as the
following: the ratio of drug and lipid was 1: 10, the ratio of cholesterol and lecithin was 1:8, the optimal rotary evaporation temperature
was 40 ℃, and the optimal sonication time was 3 minutes. The mean entrapment efficiency of the optimal PP1 liposome was（63.27±
3.32）%. The mean diameter of PP1 liposome was（157.73± 9.74）nm, and the Zeta potential was（-4.74± 0.44）mV.Conclusion:The
prepared PP1 liposome has following advantages: high encapsulation efficiency, uniform shape, even diameter and good reproduction
quality, which can provide experimental foundation for the further study of the ophthalmic drug release system. |
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