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目的：通过颈静脉注射外源性nesfatin-1，观察营养性肥胖大鼠摄食、体重、胃排空率的变化情况。方法：营养性肥胖大鼠造模成功后，各组大鼠行颈静脉插管手术，术后所有大鼠分为四组，正常对照组及肥胖对照组大鼠注射0.9%生理盐水，正常给药及肥胖给药组大鼠注射外源性nesfatin-1（100 滋g·kg-1），连续颈静脉给药7 d，期间记录各组大鼠摄食量以及体重，给药结束后采用灌胃酚红法测定大鼠胃排空率。结果：用高脂饲料连续饲养大鼠7天，正常对照组和正常nesfatin-1组大鼠的Lee’s指数分别为314.22 和314.44，肥胖对照组和肥胖nesfatin-1 组大鼠的Lee’s指数分别为318.22 和319.03，肥胖差异显著(T-test, P<0.01)，造模成功。连续给药7 d 后，给药组摄食量和体重与对照组相比明显降低，但肥胖给药组摄食量及体重下降较正常给药组更加明显。胃排空率与胃排出酚红量是成负相关的，实验中正常对照组和正常给药组的胃排空率分别是64.71± 4.51 和46.47± 3.20，而肥胖对照组和肥胖给药组大鼠的胃排空率分别是75.67± 2.47 和50.88± 3.07，因此高剂量给予nesfatin-1 能显著降低大鼠的胃排空率。结论：综上所述，长期持续外周静脉给予外源性的nesfatin-1 可以明显抑制正常及肥胖大鼠的摄食，动物体重减轻。

英文摘要:

Objective:To observe the impact of exogenous nesfatin-1 on food intake, body weight and gastric emptying after jugular vein injection in rats.Methods:Obese rats and normal rats underwent external jugular vein cannulation surgery after obese rat model was established. Afterwards, the postsurgerical rats were assigned into four groups respectively. The normal control group and obese control group were injected with 0.9% saline, while the normal nesfatin-1 group and obese nesfatin-1 group were treated with exogenous nesfatin-1 (100 ug·kg-1), and all treatments lasted for 7 days via jugular vein, during this administration period, food intake and body weight were recorded. After administration, the rate of gastric emptying was determined through oral gavage of Phenol red.Results:After a high fat diet treatment for seven days, the Lee’s indexes were 314.22± 1.80 and 314.14± 2.23 for normal control group and normal nesfatin-1 group respectively, the indexes were 318.22± 1.39 and 319.03± 1.24 for obese control group and obese nesfatin-1 group respectively, and the difference was statistically significant (T-test, P<0.01), so the obese rat model was established successfully. After a treatment for consecutive 7 days, the average food intake and body weight of nesfatin-1 group were less than the control group, and the loss of food intake and the decrease of body weight were more obvious in obese group. As well-known, the rate of gastric emptying was negatively correlated with the amount of Phenol red excreted fromthe stomach. In normal control group and normal nesfatin-1 group, the rates of gastric emptying were 64.71± 4.51 and 46.47± 3.20, respectively. For obese control group and obese nesfatin-1 group, the rates were 75.67± 2.47 and 50.88± 3.07, respectively. So it was pretty obvious that large peripheral dose of exogenous nesfatin-1 would decrease the rate of gastric emptying significantly (T-test, P<0.01).Conclusion:Large peripheral dose of exogenous nesfatin-1 could suppress the gastric emptying function, and decrease the body weight of rats.

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