陈哲 张福庆 陈兴才 王兆琪 程宇
王明霞 李省三 李继媛 苏莹 孙国新 娄峰阁.氢火焰气相色谱仪内标法测定反式脂肪酸的条件的建立及验证[J].,2015,15(18):3564-3569 |
氢火焰气相色谱仪内标法测定反式脂肪酸的条件的建立及验证 |
The Building and Validation of the Optimal conditions' Determination forLaboratory Hydrogen Flame Gas GC Method the Trans Fatty Acids byInternal Standard |
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DOI: |
中文关键词: 气相色谱仪 反式脂肪酸 反-9- 十八碳一烯酸甲酯(C18:1-9t) 反-9,12- 十八碳二烯酸甲酯(C18:2-9t,12t) |
英文关键词: GC Trans-fatty acids Anti -9 - octadecadienoic a methyl ester (C18:1-9t) Anti -9,12 - octadecadienoic acid methyl
ester (C18 :2-9t, 12t ) |
基金项目:黑龙江省自然科学基金项目(201227);黑龙江省教育厅面上项目(12531819);大学生创新创业训练项目(201311230018) |
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中文摘要: |
目的:研究氢火焰气相色谱仪(GC112A)内标法测定反式脂肪酸的实验室最佳条件。方法:采用气相色谱内标- 标准曲线法,
通过测定反式脂肪酸标准品工作液的标准曲线、重现性和分离度来确定和验证GC112A 的最佳色谱条件。结果:实验室最佳色谱
条件:毛细管柱,PC-88(100 m× 0.25 mm× 0.2 um);进样口温度:260 ℃;载气:高纯N2,压力220 kPa,流速41.0 mL/min;检测器
(FID)温度:290 ℃,氢气压力100 kPa,流速21.0 mL/min;空气压力160 kPa,流速215.0 mL/min;检测器(FID)灵敏度:1010;手动进
样,进样量:1.0 uL。结论:该方法避免了外标法(国标方法)的国产仪器局限性和操作者人工进样的不确定性,具有受仪器参数变
化的影响较小的特点,适合在基层食品检验机构推广。 |
英文摘要: |
Objective:To measure laboratory optimum conditions of trans-fatty acids in Hydrogen flame gas chromatograph
(GC112A) internal standard method.Methods:We used gas chromatography internal standard - a standard curve method to testify and
confirm the best chromatographic condition of GC112A by measuring tans-atty acids standard working liquid standard curve,
reproducibility and degree of separation.Results:Laboratory optimum chromatographic conditions: Capillary Column, PC-88 (100m×
0.25mm× 0.2 um); Inlet temperature: 260℃; The carrier gas: Purity N2, the pressure 220 kPa, flow rate of 41.0 mL/min; detector (FID)
temperature: 290 ℃ , hydrogen pressure of 100 kPa, flow rate of 21.0 mL/min; air pressure 160kPa, flow rate 215.0 mL/min; detector
(FID) Sensitivity: 1010; manual injection, injection volume: 1.0μ L.Conclusion:This method avoids domestic equipment limitations and
uncertainties of operator manual injections of the external standard method (GB method), which has a characteristics of less influence on
instrument parameters change and is suitable for promoting in primary food inspection institution. |
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