| 张梅 王宏兰 张坤 刘秀财 郭红艳 李淑艳.妊娠期糖尿病小鼠胎盘EGFR 的表达与其发病关系的研究[J].,2015,15(17):3232-3235 |
| 妊娠期糖尿病小鼠胎盘EGFR 的表达与其发病关系的研究 |
| Relationship between Expression of EGFR in Placenta of GDMMice andGestational Diabetes Mellitus |
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| DOI： |
| 中文关键词: 妊娠期糖尿病 表皮生长因子 表皮生长因子受体 |
| 英文关键词: nal diabetes mellitus Epidermal growth factor Epidermal growth factor receptor |
| 基金项目:黑龙江省教育厅科学技术研究项目（12521643） |
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| 中文摘要: |
| 目的：研究妊娠期糖尿病小鼠胎盘表皮生长因子受体（EGFR）的表达，探讨EGFR 表达与妊娠期糖尿病发病的关系。方法：
采用链脲佐菌素建立妊娠期糖尿病( gestational diabetes mellitus，GDM)小鼠模型，对照组为正常妊娠小鼠，腹腔注射等量缓冲溶
液。测定母鼠体重、血糖；计算胎鼠的存活率；测定胎鼠、胎盘重量，计算胎盘效率；RT-PCR、免疫组化分别测定GDM 组和对照组
胎盘EGFR mRNA 和EGFR 蛋白的表达。Pearson 相关性分析用于母鼠血糖与EGFR 表达的相关性分析。结果：GDM 组母鼠体重
和血糖均高于对照组（P<0.01）；GDM 组胎鼠、胎盘重量及胎盘效率均高于对照组（P<0.01）；RT-PCR和免疫组化结果显示GDM
组胎盘EGFR mRNA和EGFR蛋白的表达与对照组相比差异有统计学意义（P<0.01）。GDM 组小鼠血糖值与其胎盘EGFR 的表
达具有相关性（r=0.582, P<0.05）。结论：GDM导致胎盘EGFR 表达升高，EGFR 并不是GDM的发病因素，EGFR 是预防GDM的
潜在靶点。 |
| 英文摘要: |
| Objective:To study the relationship between the expression of EGFR in placenta of gestational diabetes mellitus
(GDM) mice and gestational diabetes mellitus.Methods:Streptozotocin was used to induce GDM in mice, the control group (normal
pregnancy) was given the same dose buffer solution. mother’s blood glucose and body weight was obtained. The weight of fetal mice and
placenta were determined after birth, then placental efficiency was measured immediately. The expression of EGFR mRNA and protein
in placenta were determined by RT-PCR and immunohistochemistry. The relationship between expression of EGFR and mother’s blood
glucose were analyzed by Pearson correlation analysis.Results:Mother’s blood glucose and body weight in GDM group were higher
than that of control group (P<0.01); The weight of fetal mice and placenta and placental efficiency in GDM group were significantly
higher than that of control group (P<0.01). The expression of EGFR mRNA and protein had significant difference between GDMgroup
and control group (P<0.01), with statistical significance. There was a correlation between mother’s blood glucose and the expression of
EGFR (r=0.582, P<0.05).Conclusion:GDM is the reason of increased expression of EGFR in placenta, but EGFR is not pathogenetic
factors of GDM. EGFR is the potential target for prevention. |
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