| 刘蕊 周健 李宝海 陈媛媛 李东旭.高浓度葡萄糖诱导人晶状体上皮细胞发生EMT[J].,2015,15(17):3212-3215 |
| 高浓度葡萄糖诱导人晶状体上皮细胞发生EMT |
| High Glucose Induced EMT of Human Lens Epithelial Cells |
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| DOI： |
| 中文关键词: 葡萄糖 人晶状体上皮细胞 上皮- 间质转分化 |
| 英文关键词: Glucose Human lens epithelial cells Epithelial-to-mesenchymal transition |
| 基金项目:国家自然科学基金项目(81370998)；陕西省科技统筹创新工程项目（2012KTCQ03-03） |
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| 中文摘要: |
| 目的：观察高浓度葡萄糖诱导人晶状体上皮细胞发生上皮- 间质转分化（epithelial-to- mesenchymal transition, EMT）。方法：
将人晶状体上皮细胞HLE-B3 系分别培养在正常葡萄糖浓度（5.5 mmol /L）DMEM 培养基和高浓度葡萄糖（35.5 mmol /L）的
DMEM培养基中24 小时，于培养的0 h、3 h、6 h、12 h、24 h在倒置显微镜下观察细胞形态学变化，采用免疫荧光染色检测晶状体
上皮细胞中EMT 相关蛋白E-cadherin 及alpha-SMA 的表达变化。结果：与正常糖浓度组相比，随着时间的延长高糖组细胞逐渐丢失
上皮细胞形态，细胞变细、变长，向纤维细胞的形态转变；同时随着时间的延长，高糖组晶状体上皮细胞中E-cadherin 染色的荧光
强度在各时间点均低于正常糖浓度组，而alpha-SMA的荧光强度却明显高于正常糖浓度组，在6 h和12 h时差异显著，有统计学意
义(P＜0.01)。结论：高浓度葡萄糖诱导人晶状体上皮细胞发生上皮- 间质转分化。 |
| 英文摘要: |
| Objective:To investigate the effects of high glucose on epithelial-to-mesenchymal transition of human lens epithelial
cells (HLEs).Methods:HLE-B3 cells were cultured in medium DMEM with normal glucose (5.5 mmol /L) or high glucose (35.5 mmol
/L)．After incubation of 0 h, 3 h, 6 h, 12 h and 24 h, the morphological changes of HLEs were observed under the inverted microscope.
The alterations of the expression level of E-cadherin and a-SMA in the HLEs were detected by immnofluorescence staining.Results:After high glucose induction, the morphology of HLE-B3 cells changed from polygon to spindle shape. Meanwhile, immnofluorescence
staining showed that the fluorescence intensity of E-cadherin was lower in the high glucose group than that in the normal glucose group at
all time points, and the expression of alpha-SMA was higher in the high glucose group than that in the normal glucose group, with significant
differences at 6 h and 12 h time points (P＜0.01).Conclusion:High glucose may induce epithelial-to-mesenchymal transition in human
lens epithelial cells. |
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