| 刘泽洲 许可嘉 张天娇 易小烈 赵福建 杨美娟 牛建昭 李健.雷公藤甲素诱导小鼠急性肝损伤的形态学研究[J].,2015,15(17):3207-3211 |
| 雷公藤甲素诱导小鼠急性肝损伤的形态学研究 |
| The Morphological Study on Acute Liver Injury of MiceInduced by Triptolide |
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| DOI： |
| 中文关键词: 雷公藤甲素 小鼠 急性肝损伤 形态学 自噬性凋亡 |
| 英文关键词: Triptolide Mice Acute liver injury Morphology Autophagic cell death |
| 基金项目:国家自然科学基金项目（81273884） |
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| 中文摘要: |
| 目的：观察雷公藤甲素诱导小鼠急性肝损伤的形态学特征，为进一步研究雷公藤甲素肝毒性的病理特点和毒理机制提供基
础。方法：昆明种小鼠以雷公藤甲素LD50 剂量（0.8 mg/kg）水溶液灌胃，分别于给药12 h及24 h后取肝组织，制备石蜡切片、冰冻
切片，行常规HE 染色、PCNA 染色、TUNEL染色及光镜观察。部分肝组织经戊二醛固定、制备超薄切片，行透射电镜下观察。
PCNA 及TUNEL染色结果采用图像分析软件进行定量分析及统计学处理。结果：0.8 mg/kg雷公藤甲素灌胃后12 h 即可诱导肝
组织炎细胞浸润、结构破坏、肝细胞坏死及代偿性增生。透射电镜下可见肝细胞内细胞骨架结构异常、细胞器大量脱落、自噬体明
显增多。PCNA 及TUNEL染色结果表明，雷公藤甲素可诱导肝细胞出现显著的增殖及凋亡。结论：雷公藤甲素可诱导肝组织炎性
反应发生，同时伴随肝细胞凋亡、坏死及代偿性增生。推测肝细胞自噬性凋亡是雷公藤甲素诱导急性肝损伤的关键病理环节。 |
| 英文摘要: |
| Objective:To observe the morphological characteristics of acute liver injury induced by triptolide in mice，and provide
the basis of pathological characteristics and toxicological mechanisms of triptolide in liver for our further research.Methods:Kunming
mice were given triptolide orally according to median lethal dose (LD50), and dissected after 12 and 24 hours separately. Liver tissue
were detected by HE, PCNA and TNUEL staining and transmission electron microscope. Then images were analyzed by image analysis
software and the data was analyzed by statistical software.Results:After treated with 0.8 mg/kg of triptolide for 12 hours, liver tissue was
inflamed and destroyed, hepatocyte necrosis and compensatory hypertrophy can be observed. The transmission electron microscope images
show the cytoskeleton was abnormal, organelles were dropped and autophagosomes were increased.Conclusion:Triptolide can induce
inflammatory reaction, accompanied by apoptosis, necrosis and compensatory hyperplasia in liver. We speculated that autophagic
cell death induced by triptolide may be the key pathological factor for acute liver injury. |
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