| 唐亚为 陈晶 何晓霞 时永全 周新民 韩英 韩者艺.不同浓度胆汁酸盐对人肝胆管癌细胞凋亡的影响[J].,2015,15(15):2845-2847 |
| 不同浓度胆汁酸盐对人肝胆管癌细胞凋亡的影响 |
| Effect of Different Concentrations of Bile Acid Salt on the Apoptosis inHuman Biliary Cancer Cells |
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| DOI： |
| 中文关键词: 胆管癌细胞 胆汁酸盐 细胞凋亡 |
| 英文关键词: Biliary cancer cells Bile salts Cell apoptosis |
| 基金项目:国家自然科学基金项目（81170372） |
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| 中文摘要: |
| 目的：探讨不同浓度胆汁酸盐对人肝胆管癌细胞RBE 凋亡的影响。方法：采用0、100 uM、500 uM、1 mM胆汁酸盐作用于人
肝胆管癌细胞后，采用流式细胞术检测细胞的凋亡率，Western-blot法检测细胞Bax、Bcl-2 蛋白的表达。结果：胆汁酸盐浓度为0、
100 uM、500 uM、1 mM 时，人胆管癌细胞的凋亡率分别为(0.7± 0.12)%、(0.9± 0.15)%、(1.4± 0.17)%、(4.8± 0.14)%，以1 mM胆汁
酸盐所致人胆管癌细胞的凋亡最明显，Bcl-2蛋白表达量逐渐减少，Bax表达量逐渐增多，以1 mM 胆汁酸盐时作用最明显，差异
具有统计学意义(P<0.05)。1 mM 胆汁酸盐处理的人肝胆管癌细胞的形态由原来的梭形变成圆形，细胞核固缩、碎裂。结论：胆汁酸
盐可以以浓度依赖性的方式导致人胆管癌细胞凋亡，以1 mM胆汁酸盐的作用最明显。 |
| 英文摘要: |
| Objective:To investigate the effect of different concentrations of bile acid salt on the apoptosis in human biliary cancer
cells.Methods:Human biliary cancer cells were indeuced by 0, 100 uM, 500 uM, 1 mMbile acid salt, flow cytometry was used to detect
the apoptotic rate, western-blot was performed to detecte the expression of Bax and Bcl-2 protein in human biliary cancer cells.Results:The the apoptotic rates of human biliary cancer cells induced by 0, 100 uM, 500 uM, 1 mM bile acid salt were (0.7± 0.12)%, (0.9±
0.15)%, (1.4± 0.17)%, (4.8± 0.14)%, 1 mM bile acid salt could obviously enhance the apoptosis in human biliary cancer cells(P<0.05).
The expression of Bax protein and Bcl-2 protein were both gradually decreased with the increase of bile acid salt concentration. Karyopyknosis
and Karyorrhexis were increased in 1 mM bile acid salt could induced human biliary cancer cells.Conclusion:Bile acid salt
could enhance the apoptosis of human biliary cancer cells in a concentration dependent manner, 1mMbile acid salt was strongest. |
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