| 孙敏 张磊 宋淑玲 陈作雷 刘英志 殷积慧.七氟烷对培养的小鼠小胶质细胞炎症因子表达的影响[J].,2015,15(15):2840-2844 |
| 七氟烷对培养的小鼠小胶质细胞炎症因子表达的影响 |
| Effect of Sevoflurane on the Expression of Inflammation Cytokine inMicroglia of Cultured Mice |
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| DOI： |
| 中文关键词: 七氟烷 神经炎症 IL-6 TNF-alpha NF-kB |
| 英文关键词: Sevoflurane Neuroinflammation IL-6 TNF-alpha NF-kB |
| 基金项目:国家青年自然科学基金项目（81300934） |
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| 中文摘要: |
| 目的：探讨七氟烷对培养的小鼠小胶质细胞中炎症因子表达的影响。方法：取新生（2~3 天）C57BL/6小鼠，分离小胶质细胞，
将其随机分为4 组（n=10）：对照组（Control）；七氟烷组（Sevoflurane）；NF-资B抑制剂组（PDTC）；NF-kB 抑制剂+七氟烷组
（PDTC+Sevoflurane）。用Drager 麻醉机向Sevoflurane 组PDTC+Sevoflurane 组培养的小胶质细胞盒内释放21%O2，5%CO2，4.1%
七氟烷的气体，用气体分析仪持续监测各组的浓度。应用Iba-1 的免疫荧光染色法对小鼠小胶质细胞进行纯度鉴定。分别在于给
七氟烷后2 h、4 h 和6 h 时采用免疫印迹分析技术检测两组小胶质细胞IL-6 和TNF-alpha的表达水平和NF-kB 的活性。
PDTC+Sevoflurane 组在给七氟烷前一小时给予PDTC，采用ELISA 技术和免疫印迹分析技术检测各组小胶质细胞IL-6 和
TNF-alpha的浓度和NF-kB 的表达。结果：免疫印迹显示七氟烷组细胞中IL-6、TNF-alpha水平和NF- kB 的激活水平升高；PDTC降低了
七氟烷作用后核内NF-kB 的表达，减弱了IL-6和TNF-alpha水平的升高作用。结论：七氟烷可通过激活NF-kB信号通路，进一步激
活培养的小鼠小胶质细胞中炎症因子的表达。 |
| 英文摘要: |
| Objective:To evaluate effect of sevoflurane on the expression of inflammation cytokine in neuroglioma cells of cultured
mice.Methods:Microglia cells abtained from newborn (2~3 days) C57BL/6 mice were separated and randomly divided into 4
groups (n=10): control group (Control); sevoflurane group (Sevoflurane); NF-kB inhibitors group（PDTC); NF-kB inhibitors group+
sevoflurane group (PDTC+Sevoflurane). Oxygen (21 %), Carbon dioxide (5 %) and sevoflurane(4.1 %) were delivered from an Drager
anesthesia machine to the microglia cells sealed in a plastic box of the two groups. The two groups are Sevoflurane group and
PDTC+Sevoflurane group in which the later group had been given PDTC 1 hour before sevoflurane treatment. A Drag gas analyzer was
used to continuously monitor the concentrations of the O2, CO2 and sevoflurane respectively. Iba-1 immunofluorescence staining method
was used to the purity identification of microglia in mice. We examined the expression of IL-6 and TNF-alpha(by Western Blotting Analysis
and ELISA)and activation of NF-kB (by Western Blotting Analysis) at 2,4 and 6h after sevoflurane treatment.Results:Sevoflurane increases
levels of IL-6 and TNF-alpha through activates NF-kB signaling pathway.Conclusion:These studies suggest that NF-kB signaling
pathway could contribute to the increasing expression of inflammation cytokine. |
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