| 金玉龙 徐莉 唐海龙 卢晓琳 王伟娜 宋凡 陈协群.丙戊酸钠协同硼替佐米对RPMI8226 细胞的促凋亡作用[J].,2015,15(14):2649-2651 |
| 丙戊酸钠协同硼替佐米对RPMI8226 细胞的促凋亡作用 |
| Pro-apoptotic Effect of Valproic Acid and Bortezomib on RPMI8226 Cells |
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| DOI： |
| 中文关键词: 丙戊酸钠 硼替佐米 RPMI8226 细胞 凋亡 |
| 英文关键词: Valproic acd Bortezomib RPMI8226cells Apoptosis |
| 基金项目:国家自然科学基金项目（81172247） |
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| 中文摘要: |
| 目的：以骨髓瘤细胞株RPMI8226 为实验对象，观察丙戊酸钠（valproic acid，VPA）和硼替佐米(bortezomib，BZ)对此细胞株
的增殖及凋亡的诱导情况。方法：实验分组如下：对照组，VPA 单药组（1.0 mmol/L），BZ 单药A 组（10.0 nmol/L），BZ 单药B 组
（20.0 nmol/L），BZ单药C组（35.0 nmol/L），联合用药A 组（VPA1.0 mmol/L+BZ10.0 nmol/L），联合用药B 组（VPA 1.0 mmol/L+BZ
20.0 nmol/L），联合用药C 组（VPA 1.0 mmol/L+BZ 35.0 nmol/L）。用MTT 技术检测细胞增殖抑制情况；流式细胞仪检测凋亡比
例。结果：丙戊酸钠与硼替佐米单用对RPMI8226细胞株细胞增殖有抑制作用，有细胞凋亡，但丙戊酸钠与硼替佐米协同用药A
组、B组、C 组增殖抑制可达75.1%及凋亡情况可达68.9%（P＜0.01)。结论：丙戊酸钠与硼替佐米协同用药后对RPMI8226 细胞增
殖抑制及诱导凋亡作用更显著，丙戊酸钠对硼替佐米有增敏作用。 |
| 英文摘要: |
| Objective:To investigate the effects of valproic acid(VPA) and bortezomib(BZ) on cell proliferation and apoptosis in
RPMI8226 cells.Methods:The drug groups were set as follows: control group; VPA alone group (1.0 mmol/L); BZ alone group A (10.0
nmol/L); BZ alone group B (20.0 nmol/L); BZ alone group C (35.0 nmol/L); combination group A (VPA 1.0 mmol/L+BZ 10.0 nmol/L);
combination group B (VPA 1.0 mmol/L+BZ 20.0 nmol/L); combination group C (VPA 1.0 mmol/L+BZ 35.0 nmol/L). The cell
proliferation was tested by MTT and the apoptotic ratio was observed by flow cytometry.Results:Both valproic and bortezomib alone
can inhibit the cell proliferation and induce the apoptosis in RPMI8226 cells; the cell death ratio was increased by 75.1%, and the
apoptotic ratio was increased by 68.9%; when the cells were treated by valproic and bortezomib together, the effect was more obvious.Conclusion:The effect of valproic and bortezomib used together was significantly enhanced on promoting cell apoptosis and inhibiting
the cell proliferation of RPMI8226 cells , suggesting that valproic plays a role. |
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