| 伍晓晓 卫国 关月 闫佳佳 段佳林 殷英 郭超 朱艳荣 奚苗苗 文爱东.大黄酸和迷迭香酸单用及配伍对慢性肾脏疾病的保护作用研究[J].,2015,15(13):2432-2435 |
| 大黄酸和迷迭香酸单用及配伍对慢性肾脏疾病的保护作用研究 |
| Renal Protection of Rhein and Rosemarinic Acid Used Alone or inCombination by Inhibiting Cell Apoptosis |
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| DOI： |
| 中文关键词: 大黄酸 迷迭香酸 肾组织 抗凋亡 |
| 英文关键词: Rhein Rosemarinic acid Chronic renal disease Anti-apoptosis |
| 基金项目:国家自然科学基金项目（81470174；81303264）；第四军医大学西京医院学科建设助推计划（XJZT13M05） |
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| 中文摘要: |
| 目的：探讨大黄酸和迷迭香酸单用及配伍通过抗凋亡对5/6 肾切除(5/6Nx)大鼠的保护作用。方法：采用5/6 肾切除手术制作
慢性肾损伤模型，将30 只雄性SD 大鼠随机分为假手术组、模型组、大黄酸（150 mg/kg/day）治疗组、大黄酸（75mg/kg/day）+ 迷迭
香酸（75 mg/kg/day）治疗组和迷迭香酸（150 mg/kg/day）治疗组。给药1 个月后处死大鼠，测量各组大鼠血清肌酐(Scr)和尿素氮
(BUN)水平，通过HE 染色观察肾组织形态学变化，通过TUNEL 染色和测量肾组织中Bax、Bcl-2 和cleaved caspase 3 的表达检测
细胞凋亡。结果：与模型组相比，大黄酸和迷迭香酸单用及配伍都可显著降低血清肌酐和尿素氮（P＜0.05）水平，改变组织形态学
的变化和抑制肾脏细胞凋亡，且配伍的效果优于单用。结论：大黄酸和迷迭香酸配伍发挥肾保护作用明显优于单用，其作用机制
与抗凋亡作用相关。 |
| 英文摘要: |
| Objective:To investigate the renal protection effect of rhein and rosemarinic acid used alone or in combination, on 5/6
nephrectomy (5/6Nx) rats by inhibiting apoptosis response in rats.Methods:The model of chronic renal disease was induced by 5/6
nephrectomy. 30 male SD rats were divided randomly into 5 groups: sham, model, rhein （150 mg/kg/day）, rosemarinic acid（150
mg/kg/day）and rhein （75 mg/kg/day）+ rosemarinic acid（75 mg/kg/day）. All rats were killed after one month. The levels of Scr and
BUNwere measured, pathological changes were observed by HE stain. The apoptosis of renal was measured by TUNEL stain and the
protein expression of Bax, Bcl-2 and Cleaved caspase-3.Results:Compared with the model rats, the levels of Scr and BUN, pathological
changes and the apoptosis were significantly decreased after treatment with rhein, rosemarinic acid and rhein+ rosemarinic, but the combined
group has the better effect than alone.Conclusion:Treatment that combines rhein and rosemarinic acid were superior to treatment
with either drug alone. The mechanismmay be related to its anti-apoptosis. |
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