| Objective:To observe the effect of MC3T3-E1 pre-osteoblasts at different culture time on mineralized nodules
morphology, and explore an experimental method which not only can save experiment time and funds, but also is convenient to observe
the morphology differences of mineralized nodules.Methods:The MC3T3-E1 pre-osteoblasts were divided into four groups according to
the cultured time (14, 21, 28, 35 days group), then they were stained by alizarin red staining at the end of the experiment, to observe the
morphological changes of the mineralized nodule under optical microscope.Results:The formation of mineralized nodule was detected
in all the four groups. With the extension of the cultured time, the staining area increased, and the density also increased. On the 14th day,
the nodules were clear, and the distance between nodules was large, and on the 21st day, the area of nodules increased, and on the 28th
day, the nodule boundary were beyond vision, while on the 35th day, the field was largely hyperchromatic, and the outline of nodule was
unclear.Conclusion:In the period of this experiment, it was suitable for MC3T3-E1 pre-osteoblasts to culture for 14 to 21 days by
alizarin red staining. Cultured for 14 days, the cell number, size and morphology can be clearly observed when it is the best time to study
the effects of different factors on the MC3T3-E1 pre-osteoblast mineralization. |
