| 刘宇1 曹清心2 石屹城1 崔玲1 陈启1 于曼丽1 赵仙先1.自噬对高糖诱导的人冠状动脉内皮细胞凋亡的保护作用[J].,2015,15(12):2201-2204 |
| 自噬对高糖诱导的人冠状动脉内皮细胞凋亡的保护作用 |
| Protective Effect of Autophagy on Human Coronary Artery Endothelial CellApoptosis Induced by High Glucose |
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| DOI： |
| 中文关键词: 自噬 内皮细胞 高糖 凋亡 保护 |
| 英文关键词: Autophagy Endothelial cell High-glucose Apoptosis Protective |
| 基金项目:国家自然科学基金项目（81370266；81170223）；上海市自然科学基金项目（134119a0301；13ZR1450300） |
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| 中文摘要: |
| 目的：研究自噬对高糖诱导的人冠状动脉内皮细胞凋亡的影响。方法：将人冠状动脉内皮细胞，分别用常规培养基（正常对
照组）、含30 mmol/L D- 葡萄糖的高糖培养基（高糖组）、高糖培养基合并雷帕霉素（Rapamycin，RAPA；100 nmol/L）干预（RAPA
组）和高糖培养基合并3- 甲基腺嘌呤（3-Methyladenine，3-MA，5 mmol/L）干预（3-MA 组）培养。利用CCK-8 法检测细胞生长活
力，使用流式细胞术检测细胞凋亡水平，western blot 检测细胞自噬标记蛋白（Beclin1）的表达水平。结果：（1）高糖溶液刺激内皮
细胞24 h后，细胞生长活力为正常组的55.0%（P<0.01），自噬标记蛋白Beclin1 的表达水平明显增加，凋亡水平为正常组的2.0
倍；（2 ）与高糖组相比，RAPA 组细胞生长活力明显增加，Beclin1的表达明显升高（P<0.01），凋亡水平为高糖组的70.1%；（3 ）与高
糖组相比，3-MA 组细胞生长活力明显减少，Beclin1的表达明显降低（P<0.01），凋亡水平为高糖组的1.42 倍。结论：细胞自噬可
能对高糖诱导的人冠状动脉内皮细胞具有凋亡保护作用。 |
| 英文摘要: |
| Objective:To study the protective effect of autophagy on endothelial cells apoptosis induced by high glucose.Methods:Human coronary artery endothelial cells cultured by conventional media (Control group), conventional media contraining 30
mmol/L D-glucose (High-glucose group), high-glucose media plus 100 nmol/L Rapamycin (RAPA group), or high-glucose media plus
5mmol/L 3-Methyladenine (3-MA group). Cells vitality and apoptosis were measured by CCK-8 assay and flow cytometry. The
autophagy marking protein (Beclin1) expression was determined by western blot.Results:(1) Endothelial cell viability decreased
significantly after high-glucose (30 mmol/L) stimulation, with 55.0%to the control group (P<0.01). Beclin1 expressed increased, and the
level of cell apoptosis was significanly increased, with 2 times to the control group. (2) Compared with high-glucose group, the cell
viability and the beclin1 expression of RAPA group were both increased (P<0.01). The level of cell apoptosis was significanly decreased,
with 70.1% to the high-glucose group. (3) Compared with high-glucose group, the cell viability and the beclin1 expression of RAPA
group were both decreased (P<0.01). The level of cell apoptosis was significanly increased, with 1.42 times to the high-glucose group.Conclusion:Cell autophagy might have an apoptosis-protective effect on the human coronary artery endothelial cells induced by
high-glucose. |
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