| 龚伟明 莫小辉 陆海空 钱伊红 柴喆 顾昕 管志芳.运用PCR 扩增技术检测解脲脲支原体生物群的临床应用[J].,2015,15(10):1848-1850 |
| 运用PCR 扩增技术检测解脲脲支原体生物群的临床应用 |
| Clinical Significance of Using 16S rRNA Gene for Detection the Biovars ofUreaplasma Urealyticum (Uu) by Polymerase Chain Reaction |
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| DOI： |
| 中文关键词: 脲脲原体 聚合酶链反应 16S rRNA |
| 英文关键词: Ureaplasma urealyticum Polymerase chain reaction 16S rRNA |
| 基金项目:上海市卫生局科研计划课题资助项目（20134324） |
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| 中文摘要: |
| 目的：探讨运用酶链聚合反应( PCR)技术检测泌尿生殖道解脲脲原体(Uu)的生物群，分析Uu 生物群与临床症状的相关性。
方法：以支原体16S rRNA保守区域基因为扩增靶序列设计引物，采用PCR 方法扩增Uu 16S rRNA基因检测125 例临床标本，并
将检测结果与临床症状进行相关性分析。结果：PCR法检出Uu 阳性率44.8%，其中35 例Uu 生物1群阳性，其中有16 例有症状；
27 例Uu 生物2 群阳性，其中有18 例有症状。Uu 生物1 群感染与症状的相关性无统计学差异（P>0.05），而Uu 生物2 群感染与
症状相关性有统计学差异（P<0.05）。结论：PCR 检测Uu 16S rRNA 基因可用于Uu 生物群的检测，Uu 生物2 群可能是非淋菌性尿
道炎（NGU）的一个致病菌, 而Uu 生物1 群与NGU无明显相关性。 |
| 英文摘要: |
| Objective:To evaluate the association between the biovars of Ureaplasma urealyticum (Uu) infection and the
symptoms of the patients.Methods:125 swab specimens from patients were collected and were detected using 16s rRNA gene by PCR.
The relation between the biovars of Uu and symptoms was analyzed.Results:The positive rates of Uu, the parvo biovar and the T960
biovar were 44.8%, 28.0%and 21.6%, respectively. There was a significant association between infection of the T960 biovar and signs of
urethral inflammation. No significant relation was found between patients with the parvo biovar of Uu and clinical signs.Conclusion:PCR for 16s rRNA gene could be used for the detection of the biovars of Uu, and T960 biovar of Uu could cause urethritis in patients
with nongonococcal urethritis (NGU). |
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