| 卢晓琳 王欢 杨磊 周子凌 金玉龙 丁寅.甲状旁腺激素对成骨细胞中ClC-3 氯通道表达及成骨分化影响的研究[J].,2015,15(10):1830-1835 |
| 甲状旁腺激素对成骨细胞中ClC-3 氯通道表达及成骨分化影响的研究 |
| Effect of PTH on the ClC-3 Expression and Osteodifferentiation inMC3T3-E1 Cells |
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| DOI： |
| 中文关键词: 甲状旁腺激素 ClC-3 氯通道 成骨分化 |
| 英文关键词: Parathyroid hormone ClC-3 chloride channel Osteodifferentiation |
| 基金项目:国家自然科学基金青年科学基金项目（81200648） |
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| 中文摘要: |
| 目的：观察甲状旁腺激素(PTH)对成骨细胞中ClC-3 氯通道表达及成骨分化影响，初步探索ClC-3 介导PTH在细胞成骨分
化中的作用。方法：采用10-8 M、10-9 M、10-10MPTH 持续刺激和间断刺激MC3T3-E1 细胞72 h后，通过CCK-8试剂盒法检测
MC3T3-E1 细胞的增殖情况，Real-Time PCR法检测MC3T3-E1 细胞中Clcn3及成骨相关基因Alp、Runx2的表达情况，免疫荧光
法检测10-9MPTH 不同给药方式下对ClC-3蛋白表达的影响。结果：经不同浓度PTH 连续和间断处理72 h后，结果显示10-9M
PTH间断刺激的MC3T3-E1 细胞的增殖能力最强，且其Alp、Runx2 mRNA 表达均高于10-8M 组和10-10M 组(P<0.05)，而相同浓
度间断刺激的MC3T3-E1 细胞成骨相关基因的表达均高于持续刺激组，以10-9 M 间断刺激组差异最显著(P<0.05)，而10-8 M和
10-10 M 均无统计学差异(P>0.05)，10-9MPTH 刺激的MC3T3-E1 细胞中ClC-3 蛋白表达也显著增加(P<0.05)。结论：成骨细胞的
ClC-3 氯通道能够响应PTH 的刺激发生变化，并伴随着成骨相关基因Alp、Runx2 表达的增强。 |
| 英文摘要: |
| Objective:To observe the effects of parathyroid hormone (PTH) on the expression of ClC-3 and osteogenic genes
expression in MC3T3-E1 cells, and investigate the role of CIC-3 in osteoblast differentiation through PTH.Methods:10-8M, 10-9 M, 10-10
M PTH were used to continuously and intermittently stimulate the MC3T3-E1 cells for 72 h, then Cell Counting Kit-8 was used
performed to detect the proliferation of MC3T3-E1 cells, Real-Time PCR was applied to measure the genes expression levels of Alp,
Runx2 and Clcn3, immunofluorescence analysis was used to detect the expression of ClC-3 protein.Results:After the continuous and
intermittent stimulation of different concentrations of PTH for 72 h, the proliferation of MC3T3-E1 cells treated by intermittent
stimulation of 10-9MPTH was the most strongest, in which the Alp, Runx2 mRNA expressions were both significantly higher than those
of 10-8M and 10-10M PTH (P<0.05). The osteogenic genes expressions of intermittent stimulation with the same concentration of PTH
were all significantly higher than those of the continuous stimulation, which were the highest in intermittent stimulation with 10-9MPTH
(P<0.05), while no significant difference was found between 10-8 M and 10-10 M group (P>0.05). The ClC-3 protein expression in
MC3T3-E1 cells stimulated by 10-9M PTH was also obviously increased (P<0.05).Conclusion:PTH stimulation could promote the
ClC-3 expression in MC3T3-E1 cells, along with the enhancement of osteogenic genes (Alp, Runx2) expression. |
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