| 张玮 王巧玲 逄明杰 祝海 郭菲菲 孙向荣 公衍玲 徐珞.下丘脑Nesfatin-1/NUCB2对糖尿病小鼠摄食的影响[J].,2015,15(8):1440-1443 |
| 下丘脑Nesfatin-1/NUCB2对糖尿病小鼠摄食的影响 |
| The Effects of Hypothalamic Nesfatin-1/NUCB2 on Feeding in Diabetes Mice |
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| DOI： |
| 中文关键词: 摄食过多 Nesfatin-1/ NUCB2 下丘脑 糖尿病 小鼠 |
| 英文关键词: Hyperphagic feeding Nesfatin-1/nucleobindin-2 Hypothalamus Diabetes Mice |
| 基金项目:国家自然科学基金项目（31071014；81100260；81270460；81300281；81470815）；
青岛市科技局项目（13-1-4-170-jch，11-2-3-3-(2)-nsh；14-2-3-3-nsh） |
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| 中文摘要: |
| 目的：探讨下丘脑神经肽NUCB2与Tsumura Suzuki（TS）多基因突变2 型糖尿病（T2DM）小鼠摄食过多的关系。方法：将动
物分为Tsumura Suzuki 糖尿病（TSD）小鼠、正常小鼠；监视器监测小鼠摄食量；分析血生化指标；定量RT-PCR 分析摄食相关神经
肽mRNA 表达水平；放射免疫分析法检测nesfatin-1 蛋白水平。结果：与年龄匹配的TSN 小鼠相比，TSD 小鼠在1 月龄就存在体
重增加（P＜0.05）和高瘦素血症（P<0.05），3-12 月龄出现贪食（P＜0.05）、高血糖（P＜0.05）、高血脂（P＜0.05）和高胰岛素血症（P<0.
05），且3-12 月龄时厌食肽nesfatin-1 前体核连蛋白2（NUCB2）mRNA 和nesfatin-1 蛋白水平均显著降低（P<0.05~0.01）；TSD 小
鼠下丘脑甘丙肽、黑色素浓集素、神经肽Y 及前黑素细胞皮质素原mRNA水平也有显著改变（P<0.05）。结论：下丘脑NUCB2 介
导信号通路破坏可能导致TSD 小鼠摄食过多。 |
| 英文摘要: |
| Objective:To discuss the relationship between hypothalamic neuropeptide NUCB2 and hyperphagic feeding in
Tsumura Suzuki Diabetes (TSD) mice, a model of type 2 diabetes with polygenic abnormalities.Methods:Animals can be divided into
Male Tsumura Suzuki Diabetes (TSD) mice, Tsumura Suzuki normal (TSN) mice. Food intake in mice was monitored and blood
chemistry was analyzed. Quantitative real-time polymerase chain reaction (PCR) assay was used to measure the expression level of
feeding-related neuropeptide mRNA; radiation immunoassay was used to detect the protein level of nesfatin-1.Results:TSD mice
showed an increase in body weight (P<0.05) and hyperleptinemia (P<0.05) from 1 month of age and hyperphagic feeding (P<0.05),
hyperglycemia (P<0.05), hyperlipidemia (P<0.05) and hyperinsulinemia (P<0.05) from 3 to 12 months of age compared with
age-matched non-diabetic control TSN mice. The mRNA and protein levels of nucleobindin-2 (NUCB2), the precursor of the
anorexigenic neuropeptide nesfatin-1, was significantly decreased (P<0.05~0.01) in the hypothalamus of TSD mice compared with that in
TSN mice from 3 to 12 months of age. The mRNA levels of galanin, melanin-concentrating hormone, neuropeptide Y, and
pro-opiomelanocortin were significantly changed (P<0.05) in the hypothalamus in TSD mice at several time points.Conclusion:The
results suggest that the disrupted control of hypothalamic NUCB2-mediated signaling may contribute to hyperphagic feeding in TSD
mice. |
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