| 刘颖 栾晓 逄明杰 祝海 郭菲菲 孙向荣 公衍玲 徐珞.下丘脑Nesfatin-1抑食效应及机制研究[J].,2015,15(8):1424-1428 |
| 下丘脑Nesfatin-1抑食效应及机制研究 |
| Nesfatin-1 in the Hypothalamus Antifeedant Effect and MechanismStudy |
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| DOI： |
| 中文关键词: Nesfatin-1 摄食 促甲状腺激素释放激素 组胺 促肾上腺皮质激素释放激素 |
| 英文关键词: Nesfatin-1 Feeding behavior CRH Histamine TRH |
| 基金项目:国家自然科学基金项目（31071014；81100260；81270460；81300281；81470815）； |
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| 中文摘要: |
| 目的：探讨下丘脑nesfatin-1 与组胺信号通路间的相互作用及对摄食的影响。方法：采用第三脑室置管、药物注射、免疫组化、
ELISA 等方法，观察氟甲基组氨酸(FMH)、琢螺旋促肾上腺皮质激素释放激素(CRH)和促甲状腺激素释放激素(TRH)对Nesfatin-1
诱导的抑制摄食的影响，以及Nesfatin-1 与组胺信号通路相互影响调控摄食机制。结果：第三脑室注射nesfatin-1 可显著减少大鼠
摄食量，而第三脑室内预先注射FMH，nesfatin-1 抑制摄食效应明显减弱，但FMH 本身并不影响大鼠夜间摄食量。第三脑室注射
nesfatin-1，可显著增加优降宁诱发的PVN、腹内侧核(VMH）、结节乳头核(TMN)内t-MH 的积累；但腹腔注射nesfatin-1 没有引起
大鼠摄食改变，t-MH蓄积也无显著变化。第三脑室注射琢螺旋CRH或抗TRH 血清均可显著减弱nesfatin-1 的抑食效应，而琢螺
旋CRH、抗TRH 血清本身并不显著影响大鼠摄食量。第三脑室注射nesfatin-1 可显著增加下丘脑PVN 内CRH和TRH 水平，且
nesfatin-1 可显著增加优降宁诱导的PVN、VMH 和TMN 内t-MH 的表达，而琢螺旋CRH 或抗TRH 血清可显著抑制nesfatin-1
诱导的PVN、VMH 和TMH 内t-MH 的蓄积。第三脑室注射组胺可显著增加大鼠下丘脑PVN内nesfatin-1 含量，但LH、VMH、
TMN 以及血浆内nesfatin-1 水平无显著改变。免疫组化研究显示，PVN内有nesfatin-1 和H1-R 免疫反应阳性神经元，且部分神经
元共存。结论：Nesfatin-1 的抑食效应可能与下丘脑组胺信号通路介导。 |
| 英文摘要: |
| Objective:To examine the interaction of nesfatin-1 in hypothalamus and histamine signaling pathways and the effect
of nesfatin-1 in hypothalamus on the feeding behavior in rats.Methods:Third ventricle catheter, drug injection, immunohistochemistry,
ELISA methods were used to observe the effects of FMH, CRH and TRH on the inhibition of feeding induced by nesfatin-1. In addition,
the interaction of nesfatin-1 and histamine signaling pathways on the regulation of feeding.Results:Third ventricle nesfatin-1 had
significantly reduced food intake in rats, while the feeding inhibition third of the third intraventricular injection of FMH, advance
nesfatin-1 on rats reduced significantly, but the FMH itself does not affect night food intake in rats. The third ventricle injection of
nesfatin-1, can significantly increase the pargyline induced t-MH accumulation in PVN, VMH, TMN; but the intraperitoneal injection of
nesfatin-1 did not induce rat feeding change, t-MH accumulation also showed no significant changes. Third ventricle injection of alpha
helix CRH serum can significantly reduce TRH nesfatin-1 feed inhibitory effect, while alpha helix CRH, TRH serum itself does not
significantly affect food intake in rats. The third ventricle injection of nesfatin-1 can significantly increase the CRH and TRH levels in
PVN, and nesfatin-1 can significantly increase the pargyline induced t-MH expression in PVN, VMH and TMN t-MH. Alpha helical
CRH or anti-TRH serum can inhibit the nesfatin-1 induced accumulation of t-MH in PVN, VMH and TMH. Third ventricle injection of
histamine can significantly increase the content of nesfatin-1 in PVN of hypothalamus, but the content of nesfatin-1 in LH and VMH,
TMN and plasma had no significant changes. Immunohistochemical study showed that in PVN and H1-R nesfatin-1 immune response
positive neurons, and part of the coexistence of neurons.Conclusion:The feeding inhibitory effect of nesfatin-1 may be related to the
hypothalamus histamine signaling pathway mediated. |
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