| 李红星 彭肖肖 申汉威 李俊卿 杨孔宾.肌肉注射人神经生长因子基因逆轴突传递修复神经损伤的研究进展[J].,2015,15(4):788-791 |
| 肌肉注射人神经生长因子基因逆轴突传递修复神经损伤的研究进展 |
| Intramuscular Injection of Human Nerve Growth Factor Gene RetrogradeAxonal Transport Repair Nerve Injury |
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| DOI： |
| 中文关键词: 神经生长因子 基因治疗 肌肉注射 逆轴突传递 神经损伤 |
| 英文关键词: Nerve growth factor(NGF） Gene therapy Intramuscular injection Retrograde axonal transport Nerve injury |
| 基金项目:国家自然科学基金项目（81272788）；哈尔滨市科技局创新人才基金项目（2012RFXXS018） |
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| 中文摘要: |
| 神经生长因子（NGF）促进中枢及外周神经系统神经元细胞存活、分化、轴突再生等重要作用已得到临床的广泛证实。目前临
床上主要以局部或肌肉注射NGF蛋白的方式对神经系统的损伤进行治疗。但NGF半衰期短、局部应用副作用大、费用昂贵、难以
透过血脑屏障等缺点而限制临床应用。长期以来，科研工作者致力于寻求一种理想的途径或方法以克服这一缺陷。随着基因工程
技术的飞速发展，研究人员发现通过骨骼肌肌肉注射途径，以非病毒载体介导外源的NGF基因体内表达并逆轴突传递到神经损
伤部位，有望解决这一难题。本文将就NGF及受体的基本结构和特性、逆轴突传递的机制、非病毒载体结合骨骼肌肌肉注射的基
因治疗等方面进行总结和阐述。 |
| 英文摘要: |
| The nerve growth factor (NGF) plays a critical role in the central and peripheral nervous system. NGF promotes
proliferation, differentiation and axonal regeneration of neurons. The administration of NGF protein into injured nerves has been shown
to promote nerve repair and enhance functional restoration following nerve damage. However, local delivery of NGF has the problem of
its short half-life, more side effects and through the blood-brain barrier difficultly. To solve these problems, many groups are working on
improving NGF delivery to the nervous system via several methods. Following the rapid development of genetic engineering technology.
The researchers found that can be solved via skeletal muscle intramuscular non-viral vector mediated exogenous NGF gene and
retrograde axonal transport to the nerve injury site. The purpose of this review was to make a brief description about the basic structure
and characteristics of NGF and its receptors, mechanism of the retrograde axonal transport and non-viral vector combined with skeletal
intramuscular mediates gene therapy. |
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