| Objective:To compare the clinical significances of enzyme cut signal amplification method (Cervista) and diversion
hybrid gene chip technology (HybriMa) in the diagnosis of Cervical intraepithelial neoplasia of grade 2 or above(CIN2+).Methods:288
patients (aged 20～65) underwent cervical cytology for the detection of ASCUS (atypical squamous cells of uncertain meaning) and admitted
from March 2012 to January 2013 in the first affiliated hospital of Harbin medical university outpatient were selected, the finished
specimens of cervical cytology were used for the detection of residual with Cervista technology, diversion and hybridization, as well as
cervical biopsy under colposcope. A gold standard diagnosis based the pathological diagnosis results, the sensitivity, specificity and ROC
curves of Cervista and HybriMa technology in the diagnosis of CIN2+ were compared.Results:In the 288 patients, the positive rate of
Cervista technology and diversion type hybrid gene chip technology in the detection of high-risk HPV were 49.31% and 51.39% respectively
(P>0.05), the sensitivity of Cervista and HybriMa technology in the diagnosis of CIN2+ were 95.65 and 91.30%, respectively(P>0.
05), the specificity were 59.50%and 56.20%, respectively (P>0.05), the positive predictive value were 30.99%and 28.38%, respectively
(P>0.05), negative predictive value were 98.63%and 97.14%, respectively (P>0.05), and the area under ROC curve were 0.776 and 0.738,
respectively.Conclusion:The clinical significance of Cervista technology in the diagnosis of CIN2+ was equal to the HybriMa technology. |
