| 丁丽 张绍维 赵文洲 罗晶 王巍 张锦.GLP-1 对人脐静脉内皮细胞释放NO的影响及机制的研究[J].,2015,15(4):632-634 |
| GLP-1 对人脐静脉内皮细胞释放NO的影响及机制的研究 |
| Effects and Mechanismof Glucagon like Peptide-1 on Nitric OxideRelease in Human Umbilical Vein Endothelial Cells |
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| DOI： |
| 中文关键词: 胰高糖素样肽-1 脐静脉内皮细胞 一氧化氮 动脉粥样硬化 |
| 英文关键词: Glucagon-like peptide 1 Human umbilical vein endothelial cells Nitric oxide Atherosclerosis |
| 基金项目:辽宁省博士启动基金项目资助（20121114） |
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| 中文摘要: |
| 目的：观察胰高糖素样肽－1(GLP-1)对脐静脉内皮细胞（HUVECs）释放一氧化氮（NO）的影响，并探讨GLP-1 受体及GLP-1
(9-36)在其中的作用。方法：分别以GLP-1、艾塞那肽、GLP-1（9-36）、GLP-1+exendin(9-39)、GLP-1+ 西格列汀、GLP-1+ 西格列汀
+exendin(9-39)孵育HUVECs ，取培养上清以硝酸还原酶法检测NO浓度。结果：GLP-1 剂量依赖性的增加HUVECs中NO 释放，
艾塞那肽和GLP-1（9-36）均可刺激NO释放，exendin(9-39)和西格列汀均可部分阻断GLP-1 引起的NO释放。结论：GLP-1 可能通
过GLP-1 受体及GLP-1（9-36）相关的途径刺激HUVECs NO 释放,发挥直接的血管保护作用。 |
| 英文摘要: |
| Objective:To investigate the effects of glucagon-like peptide-1 (GLP-1) on nitric oxide (NO) release in human
umbilical vein endothelial cells (HUVECs) and whether GLP-1 receptor (GLP-1R) and GLP-1 (9-36) are involved in mediating the
effects.Methods:HUVECs were incubated in the presence or absence of GLP-1, exenatide (a GLP-1R agonist) or GLP-1 (9-36), or
incubated in the presence of GLP-1 with or without exendin (9-39) (a GLP-1R antagonist) and/or sitagliptin (inhibitting formation of
GLP-1 [9-36]). NO content in the cell culture medium was measured by adopting nitrate reductase method.Results:GLP-1 dose-dependently
promoted NO release in HUVECs. Exenatide and GLP-1 (9-36) both increased NO release. Exendin (9-39) and sitagliptin both
partly blocked the effects of GLP-1.Conclusion:GLP-1 may have directly protective effect on endothelium by increasing NO release in
HUVECs through GLP-1R-dependent and GLP-1(9-36)-related pathways. |
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