| 位全芳 路菊 陶忠芬 黄文琪 糜建红.FXR 激动剂GW4064 对裸鼠肝癌细胞移植瘤增殖及血管生成的影响[J].,2015,15(4):607-609 |
| FXR 激动剂GW4064 对裸鼠肝癌细胞移植瘤增殖及血管生成的影响 |
| Effect of FXR Agonist GW4064 on the Proliferation and Angiogenesisof HCC Cells in Nude Mice |
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| DOI： |
| 中文关键词: 法尼酯x 受体 GW4064 白介素-8 增殖 血管生成 肝癌 |
| 英文关键词: FXR GW4064 IL-8 Proliferation Angiogenesis Hepatocellular carcinoma |
| 基金项目:国家自然科学基金项目（30901220） |
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| 中文摘要: |
| 目的：探讨研究法尼酯x 受体(FXR)激动剂GW4064 对裸鼠肝癌细胞移植瘤增殖及血管生成的影响。方法：选取人肝癌细胞
系HepG2进行体外培养，将细胞悬液接种于BALB/c 裸鼠皮下。裸鼠成瘤后，随机分为两组，分别腹腔注射DMSO和GW4064。
一周后，处死动物取肿瘤组织，通过免疫组织化学法检测肿瘤组织中Ki-67 和CD31的表达，同时计数肿瘤组织中的微血管密度
(CD31-MVD)；Western blot 法检测其FXR 和白介素-8(IL-8)的蛋白表达。结果：与对照组相比，FXR激动剂GW4064 处理组的肿
瘤组织中FXR的蛋白表达量明显增高，微血管密度CD31-MVD 值显著降低，同时Ki-67、IL-8 及CD31 的表达水平均显著降低。
结论：FXR激动剂GW4064 能显著增加FXR 的表达，抑制裸鼠肝癌细胞移植瘤的增殖及新生血管的形成。 |
| 英文摘要: |
| Objective:To investigate the effect of FXR agonist GW4064 on the proliferation and angiogenesis of hepatocellular
carcinoma (HCC)cells in BALB/c nude mice.Methods:Human hepatocellular carcinoma cell line HepG2 were cultured in vitro, then implanted
subcutaneously in nude mice. When the subcutaneous xenograft was formed, the nude mice were randomly divided into a control
group and an experimental group, which were injected with DMSO and GW4064 respectively. After one week, the tumor was separated,
the expression of Ki67 and CD31 were detected by immunohistochemical(IHC) method, the microvessel density (CD31-MVD) of tumors
were calculated, and the expression of FXR and IL-8 were detected by Western blot assays.Results:Compared with control group, the
expression of FXR increased significantly, the microvessel density CD31-MVD value decreased significantly, while the expression level
of Ki-67, IL-8 and CD31were significantly inhibited in GW4064 group.Conclusion:FXR agonist GW4064 may stimulate the expression
of FXR thereby inhibiting the cell proliferation and tumor angiogenesis of hepatocellular carcinoma cell xenografts in nude mice. |
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