| 徐小珊 侯小明 王伟 郝佩琪 朱克伦 剡海阔 黄群山 杨世华.DMBA 诱导树鼩乳腺肿瘤（中缅树鼩）[J].,2015,15(2):228-232 |
| DMBA 诱导树鼩乳腺肿瘤（中缅树鼩） |
| DMBA Induced Breast Tumors in Tree Shrews ( Tupaia Belangeri Chinese) |
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| DOI： |
| 中文关键词: 树鼩 DMBA 诱导乳腺癌 |
| 英文关键词: Tree shrew DMBA Induced breast cancer |
| 基金项目:科技部重大科学研究计划重大科学问题导向项目 (201 2CBA01300)； 教育部 " 新世纪国家优秀人才 " 支持计划(NCET-12-1078)；
国家自 然科学基金项目 (31071 279， 30871232)；云南省科技创新人才计划项目 (2011 CI009) |
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| 中文摘要: |
| 目的： 建立一个合适的乳腺癌动物模型将在研究人类乳腺癌的发生、发展、转移等方面中发挥着越来越重要的作用 。 7,12- 二
甲基苯并蒽（ 7, 1 2-dimethylbenz anthracene， DMBA）在实验中能诱导大鼠产生乳腺肿瘤。 树鼩的基因 的结构与 人类的相似程度比
啮齿类动物要高，而且树鼩的自 发性乳腺癌已经有被报道， 因 而树鼩很有可能是研究乳腺肿瘤更合适的动物模型。 因 此我们想用
致癌剂 DMBA 诱导树鼩产生乳腺肿瘤而建立树鼩的乳腺肿瘤模型。 方法： 在这个研究中， 我们采用 了 十只在分娩之后失去幼崽
的雌树鼩， 其中一半的树鼩在腰部双侧乳房的脂肪垫注射 100 mg/kg 的 DMBA， 其余的树鼩作为 对照组没有作 DMBA 处理。 对
生成的肿瘤组织进行病理切片 HE 染色的形态特点分析以及免疫组化化学法测定 Ki-67、雌激素受体、孕酮受体、人表皮生长因 子
受体 -2、 E- 钙粘蛋白、P120 连环蛋白 的表达。 结果： 通过诊断在 DMBA 处理的树鼩中， 5 分之 1 发展浸润性导管癌， 其余发展成原
位导管癌。结果还证明了 诱导出 来的乳腺肿瘤的形态学和病理学特征与 人类的浸润性导管癌相似。 结论： 结果显示我们采用
DMBA 注射失去幼崽的雌树鼩的乳腺来诱导乳腺肿瘤是有效的， 诱导出来的肿瘤组织学特征与 人的乳腺癌相似， 诱导的肿瘤组
织表达目 前人常用的乳腺癌相关分子生物学标记， 并且表达情况与 人的乳腺癌相似。 这表明了 DMBA 诱导树鼩乳腺癌可以提供
一个适合于研究人类乳腺癌发生、发展、转移和治疗的动物模型。 |
| 英文摘要: |
| Objective:The establishment of a suitable animal model for study of human breast cancer development, metastasis and
other aspects is more crucial. 7, 12-dimethylbenz anthracene (DMBA) has potential to induce experimental breast tumors in rat. Tree
shrew is a more appropriate animal for disease models due to its genetic makeup similarities with human as compared with rodent and
spontaneous breast cancers in tree shrews were already reported. Therefore, we attempt to induce breast tumor in tree shrews by DMBA
treatment.Methods:In the present study, ten female tree shrews lost their babies after delivery were employed and half of them received a
single dose of 100 mg/kg DMBA by the lumbar bilateral mammary fatty pad injection, while the others were treated without DMBA as
control. As a result of DMBA administration, one of five candidates had developed infiltrative ductal carcinoma (IDC), while others had
developed into ductal carcinoma in situ, diagnosed with morphological features of pathological sections stained by H&E and
immunohistochemistry to detect the expression of ki-67, estrogen receptor, progesterone receptor, human epidermal growth factor
receptor-2, E-cadherin, and p1 20-catenin.Results:The results demonstrated that induced breast tumors in tree shrews have shown similar
morphological and pathological characteristics with humans IDC.Conclusion:From the results of experiment, the approach we take
mammary gland injection DMBA in female tree shrew who had lost their babies after delivery is feasible. Histological features of induced
tumor are similar to human mammary tumor, the induced tumor expressed molecular biology markers of human breast cancer, and the
expression is similar to human mammary tumor. It is suggested that DMBA-induced mammary tumor model in tree shrews may provide a
proper animal model in research of its occurrence, progression, metastasis, recurrence and treatment. |
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