| 谢潇 欧龙 罗芸 王珺 郭晓东.不同浓度尼古丁对牙周膜成纤维细胞增殖及纤维结合蛋白合成的作用[J].,2015,15(2):225-227 |
| 不同浓度尼古丁对牙周膜成纤维细胞增殖及纤维结合蛋白合成的作用 |
| Study on the Effect of Nicotine of Various Concentrations on PeriodontalLigament Fibroblasts Proliferation and Fibronectin Synthesis |
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| DOI： |
| 中文关键词: 牙周 膜成纤维细胞 尼古丁 增殖 细胞周期 纤维结合蛋白 |
| 英文关键词: Periodontal ligament fibroblasts Nicotine Cell cycle Fibronectin |
| 基金项目:十一五军队保健课题（ 10BJZ03） |
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| 中文摘要: |
| 目的： 探讨不同 浓度尼古丁对人牙周膜成纤维细胞（ PDLFs）增殖及纤维结合蛋白（ Fn）合成的作用 。 方法： 不同 浓度的尼古丁
（ 50 ng/ml， 250 ng/ml， 500 ng/ml， 1 滋g/ml， 2 滋g/ml， 3 滋g/ml）作用 于 PDLFs， MTT 比色法检测细胞增殖活性， 流式细胞仪检测 细胞
周期， 酶联免疫吸附测定法（ ELISA）检测 Fn 合成含量。 结果： 不同 浓度尼古丁作用 下： 人 PDLFs 的增殖均被抑制， 且呈浓度依赖
性。 浓度为 250 ng/ml～ 3 ug/ml 的尼古丁有明显抑制人 PDLFs 增殖的作用（ P<0.05）， 3 ug/ml 尼古丁显示出 最强的抑制 增殖作用
（ P<0.01）； 人 PDLFs 的 G0-G1 期、S 期、G2-M 期与 对照组相比， G0-G1 期细胞周 期分布比例 逐渐增高， S 期和 G2-M 期逐渐降低，
差异均有统计学意义， 呈浓度依赖性； 人 PDLFs 合成 Fn 逐渐减少， 呈浓度依赖性。 浓度为 50 ng/ml～ 3 滋g/ml 的尼古丁均有明 显
抑制人 PDLFs 合成 Fn 的作用（ P<0.05），其中 3 滋g/ml 抑制作用 最强。 结论： 尼古丁抑制牙周膜细胞的增殖及 Fn 的合成， 呈浓度依
赖性， 并影响其细胞周期的进程，进而影响牙周 新附着的形成,加重牙周病的病情。 |
| 英文摘要: |
| Objective:To explore the effect of nicotine of various concentrations on periodontal ligament fibroblasts (PDLFs)
proliferation and fibronectin (Fn) synthesis.Methods:Nicotine of different concentrations (50 ng/ml, 250 ng/ml, 500 ng/ml, 1 ug/ml, 2
ug/ml, 3ug/ml) were functioned on PDLFs. MTT colorimetry was used to detect the activity of cell proliferation, flow cytometry was
used to detect cell cycle and ELISA was applied to examine the content of Fn synthesis.Results:On different concentrations, the
proliferation of human PDLFs was all inhibited under the effect of nicotine of different concentrations, and the inhibition was
concentration-dependent. Nicotine of 250 ng/ml ～ 3 ug/ml showed obvious inhibition of human PDLFs proliferation (P<0.05) with
3ug/ml reached maximum (P<0.01 ); FCM results showed that after the effect of nicotine of different concentrations, compared with
control group, the percentage of G0-G1 cell cycle of human PDLFs increased gradually and that of S and G2-M cell cycle decreased
gradually. The differences were statistically significant and concentration-dependent; Fn synthesis of human PDLFs gradually reduced
and concentration-dependent. Nicotine of 50 ng/ml～ 3 ug/ml all showed clear inhibition of Fn synthesis (P<0.05) with 3 ug/ml reached
the maximum.Conclusion:It is suggested that the nicotine could inhibit the periodontal ligament cells proliferation and the synthesis of
Fn, which affect the formation of tooth week attached, worsen the condition of periodontal disease. |
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