| 肖鹏 初明 孙泽文 杜毅聪 张明波 徐兰 初正云 陈雪 何嘉勰 丁玲昱 焦运燊 王月丹.癌睾丸抗原TFDP3 与乳腺癌上皮间质化（EMT）的相关性研究[J].,2015,15(1):1-3 |
| 癌睾丸抗原TFDP3 与乳腺癌上皮间质化（EMT）的相关性研究 |
| Correlation Studies on Cancer-testis Antigen TFDP3 andEpithelial-mesenchymal Transition in Breast Cancer |
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| DOI： |
| 中文关键词: 癌- 睾丸抗原 TFDP3 乳腺癌 上皮间质化 |
| 英文关键词: Cancer testis antigen TFDP3 Breast cancer EMT |
| 基金项目:国家自然科学基金项目（81172884）；国家基础科学人才培养基金（J1030831/ J0108）；
北京大学创新人才培养计划，北京大学医学部新教师启动基金项目（BMU0239） |
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| 中文摘要: |
| 目的：本研究探讨了癌睾丸抗原TFDP3 与乳腺癌细胞上皮间质化（epithelial-mesenchymal transition, EMT）的关系。方法：本
研究中选取了乳腺癌细胞系（MCF-10A, MCF-7, SK-BR-3 和MDA-MB-231）作为研究对象，通过Western Blot 的方法筛选获得了
TFDP3 低水平表达的乳腺癌细胞株。进一步通过质粒转染的方式构建TFDP3 过表达的细胞系模型，观察TFDP3 在EMT 中的作
用。结果：TFDP3在MCF-10A 及SK-BR-3 中不表达, 在间质化程度较高的MDA-MB-231 中高水平表达，而在上皮化程度较高的
MCF-7 中的低水平表达。MCF-7 中过表达TFDP3 后，上皮细胞标记分子E-cadherin表达下调，而间质细胞标记分子N-cadherin、
Snail、Twist及细胞骨架蛋白Vimentin 表达上调。结论：TFDP3 可以促进乳腺癌细胞发生EMT。 |
| 英文摘要: |
| Objective:To study the relationship between cancer testis antigen TFDP3 and epithelial- mesenchymal transition
(EMT) in breast cancer.Methods:In this study, the breast cancer cell lines (MCF-10A, MCF-7, SK-BR-3 and MDA-MB-231) were analyzed
by Western Blot to obtain a TFDP3 positive strain with lower expression. Then, we established a TFDP3 over-expression cell line
by plasmid transfection, and observed the role of TFDP3 in EMT.Results:TFDP3 was not detected in MCF-10A and SK-BR-3, but at a
relatively high level in MDA-MB-231 which exhibited as a mesenchymal cell line, and at a low level in MCF-7 which retains epithelial
characteristics. Once MCF-7 over-expressed TFDP3, the epithelial cell marker E-cadherin was down-regulated, and the mesenchymal cell
markers were up-regulated, including N-cadherin, Snail, Twist and cytoskeletal proteins Vimentin.Conclusion:TFDP3 might play an important
role in promoting EMT in breast cancer cells. |
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