| 苏绍娟1 刘长山2 尹青令1 李宏1 刘建华1 成敏1△.血脂康胶囊对高脂模型大鼠EPCs功能的影响[J].,2014,14(36):7037-7041 |
| 血脂康胶囊对高脂模型大鼠EPCs功能的影响 |
| The Effects of Xuezhikang on the Functions of Endothelial Progenitor CellsIsolated fromExperimental Hyperlipidemic Rats |
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| DOI： |
| 中文关键词: 内皮祖细胞 高脂血症 血脂康 生物学特性 |
| 英文关键词: Endothelial progenitor cells Hyperlipidemia Xuezhikang Function |
| 基金项目:国家自然科学基金项目（30900290；31270993）；教育部新世纪优秀人才支持计划资助（NCET-10-0922）；
山东省高等学校科技计划项目（J11LF17）；潍坊市科技发展计划（20120182；201201120；20121228） |
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| 中文摘要: |
| 目的：高脂血症可增加心血管事件的发生率，本研究降脂红曲制剂血脂康胶囊对高脂模型大鼠的内皮祖细胞（endothelial
progenitor cells，EPCs）生物学功能的影响。方法：给予Wistar大鼠高脂饲料30 d，造成高血脂大鼠模型，灌服血脂康胶囊。密度梯
度离心法分别分离正常对照组，高脂模型组和血脂康治疗组大鼠骨髓单核细胞，应用EGM-2MV 进行体外培养。以4~6 代EPCs
为靶细胞。采用Edu 标记技术、CCK-8 检测法、粘附能力测定试验、改良的Boyden 小室、Matrigel法、荧光定量RT-PCR 等方法分
别检测EPCs 增殖、粘附、迁移、体外成血管及单核细胞趋化蛋白-1( monocyte chemoattractant protein-1，MCP-1)等炎性因子的表
达。结果：高血脂模型组大鼠EPCs 的增殖、粘附、迁移及成血管能力均明显低于对照组，但炎症因子MCP-1 表达则高于对照组；
与高脂模型组EPCs 比较，血脂康可明显促进EPCs 的增殖、粘附、迁移及成血管，下调MCP-1 的表达。结论：高脂状态下大鼠
EPCs的增殖、粘附、迁移及成血管等生物学功能受损，血脂康能调整脂质代谢，改善高脂血症大鼠EPCs 功能，从而起到保护血管
内皮的功能，减少心血管疾病的发生。 |
| 英文摘要: |
| Objective:Hyperlipidemia can increase the incidence of cardiovascular events. This study is to investigate the effects
of Xuezhikang on the functions of endothelial progenitor cells （EPCs）isolated from experimental hyperlipidemic rats.Methods:Wistar
rats were fed with high fat diet for 30 d to lead to hyperlipemia, and then Xuezhikang was given by oral gavage. Bone marrow
mononuclear cells were collected from normal, hyperlipidemic or Xuezhikang treated hyperlipidemic rat by density gradient
centrifugation and cultured with EGM-2MV medium, respectively, The 4th～6th generation EPCs were chosen as target cells and cell
biological functions, such as proliferation, adhesion, migration and the ability of forming tubes were assayed with CCK-8, Edu labeling,
adhesion test, Boyden chamber assay and Matrigel respectively. The mRNA expressions of chemokines such as monocyte
chemoattractant protein-1 (MCP-1) were analyzed by real time RT-PCR.Results:The EPCs isolated from experimental hyperlipidemic
rats exhibited decreased capabilities of proliferation, adhesion, migration and tube formation. In the mean time, the gene expression of
MCP-1 was high. Xuezhikang rescued the functions of EPCs isolated from reated hyperlipidemic rat. Namely, Xuezhikang improved the
proliferation, adhesion, migration and tube formation and down-regulated the gene expression of MCP-1 in EPCs isolated from
hyperlipidemic rats.Conclusion:Hyperlipidemia damages the functions proliferation, adhesion, migration and tube of EPCs.
Xuezhikang has antiatherogenic properties and the protection to the functions of EPCs, so that it can protect the vascular endothelial
function and reduce the occurrence of cardiovascular disease. |
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