| 苏鑫 毕良佳 孟培松 胡波 刘继乐.尼古丁对牙周炎大鼠牙周膜成纤维细胞的毒性作用[J].,2014,14(35):6849-6850 |
| 尼古丁对牙周炎大鼠牙周膜成纤维细胞的毒性作用 |
| Toxic Effects of Nicotine on the Periodontal Ligament Fibroblastsof Rats with Periodontitis |
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| DOI： |
| 中文关键词: 尼古丁 牙周炎 牙周膜成纤维细胞 毒性作用 |
| 英文关键词: Nicotine Periodontitis Periodontal ligament fibroblasts Toxicity |
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| 中文摘要: |
| 目 的： 观察尼古丁对牙周炎大鼠牙周膜成纤维细胞（ PDLFs）增殖的抑制作用 及对细胞周 期分布的影响， 探讨尼古丁的细胞
毒性作用， 为口腔疾病的预防及治疗提供基础。 方法： 选取 30 只 SD 大鼠， 采用 丝线结扎联合口 内 接种细菌的方法建立牙周 炎大
鼠模型。将不同 浓度的尼古丁分别作用 于大鼠的牙周膜成纤维细胞（ PDLFs）中， 观察大鼠牙周组织的变化情况， MTT 法测定细胞
增殖活性， 分析不同 浓度尼古丁对 PDLFs 增殖的抑制作用 。 结果： 实验组大鼠牙周 组织胶原纤维束排列 紊乱， 有炎症细胞浸润； 对
照组大鼠牙龈未见红肿或出 血。 尼古丁可抑制 PDLFs 增殖， 并且呈浓度依赖性， 随着浓度的增加， 对 PDLFs 增殖的抑制 作用 增
强， 差异具统计学意义（ P<0.05）。 与 对照组相比， 不同 浓度尼古丁作用 下牙周 炎大鼠牙周 膜成纤维细胞周 期的分布率明 显不 同
（ P<0.05）。 结论： 尼古丁对牙周膜成纤维细胞增殖具有明显的抑制作用 ， 尼古丁的浓度影响牙周 组织的修复重构能力。 |
| 英文摘要: |
| Objective:To observe the effect of nicotine in various concentrations on the proliferation and cells cycle of
periodontal ligament fibroblasts (PDLFs) and to explore the toxicity of nicotine so as to make a reference to prevent and treat the
stomatological disease.Methods:Thirty SD rats were selected to establish the rats model. The different concentrations of nicotine effect
on rats respectively in the periodontal ligament fibroblasts (PDLFs). Then the changes of periodontal tissue in rats were observed, the
cells’ proliferation activity were determined by MTT and the inhibition of nicotine with different concentrations on the PDLFs’
proliferation were analyzed.Results:The periodontal tissue collagen fiber bundle of rats in the experimental group was disorder with
inflammation cells, while that of rats in the control group was normal without swelling or bleeding. On different concentrations, the
proliferation of PDLFs was all inhibited under the effect of nicotine and the inhibition was concentration-dependent(P<0.05). FCM results
showed that after the effect of nicotine of different concentrations, compared with control group, the percentage of G0-G1 cell cycle of
PDLFs increased gradually and that of S and G2-M cell cycle decreased gradually. The differences were statistically significant and
concentration-dependent (P<0.05). And with the increase of concentrations, the inhibition of PDLFs proliferation got enhanced(P<0.05).The periodontal tissue collagen fiber bundle of rats in the experimental group was disorder with
inflammation cells, while that of rats in the control group was normal without swelling or bleeding. On different concentrations, the
proliferation of PDLFs was all inhibited under the effect of nicotine and the inhibition was concentration-dependent(P<0.05). FCM results
showed that after the effect of nicotine of different concentrations, compared with control group, the percentage of G0-G1 cell cycle of
PDLFs increased gradually and that of S and G2-M cell cycle decreased gradually. The differences were statistically significant and
concentration-dependent (P<0.05). And with the increase of concentrations, the inhibition of PDLFs proliferation got enhanced(P<0.05).Conclusion:It is indicated that the nicotine can in the inhibit the proliferation of periodontal ligament fibroblasts and the concentrations
of nicotine would influence the reconstructive capability of oral tissues. |
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