| 闫小飞 宁启兰 李冬民 杨旭东 曹 伟.低浓度哇巴因对负鼠肾小管上皮细胞增殖的影响[J].,2014,14(35):6810-6813 |
| 低浓度哇巴因对负鼠肾小管上皮细胞增殖的影响 |
| Low Doses of Ouabain Stimulate Kidney Tubular Cell Proliferation underSerum Deprivation Condition Via Activation of PI3K/Akt and ERK1 /2 |
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| DOI： |
| 中文关键词: 哇巴因 OK 细胞 细胞增殖 PI3K/Akt ERK1 /2 |
| 英文关键词: Ouabain OK cell Proliferation PI3K/AKt ERK1/2 |
| 基金项目:国家自 然科学基金青年项目（81 200545） |
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| 摘要点击次数: 818 |
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| 中文摘要: |
| 目的： 用 低血清培养液来模拟肾脏供血不足的营养不良状态，研究低浓度哇巴因 对低血清培养下 OK 细胞(负 鼠肾小管上皮
细胞)增殖的影响。 方法： 用 低浓度哇巴因（ 1 -30nM）处理 0.2%血清培养下 OK 细胞， MTT 实验和 Brdu 掺入法检测哇巴因 对 OK
细胞增殖的影响； Western blot 检测 Akt 和 ERK1/2 的磷酸化水平； 用 LY294002 和 PD98059 分别 抑制 PI3K/Akt 和 ERK1 /2 蛋白
激酶活性，观察抑制 PI3K/Akt 和 ERK1/2 对哇巴因 促进 OK 细胞增殖的影响。 结果： 低浓度哇巴因（ 1-30nM）促进 OK 细胞的增
值，上调 OK 细胞中 Akt 和 ERK1/2 磷酸化水平。 用 LY294002 和 PD98059 特异抑制 Akt 和 ERK1 /2 的活化能够抑制哇巴因的促
增殖作用 。 结论： 低浓度哇巴因（ 1 -10nM）能够促进 OK 细胞的增值， PI3K/Akt 和 ERK1 /2 信号通路参与 哇巴因 对 OK 细胞促增殖
作用的调节。 |
| 英文摘要: |
| Objectivre:To study the effect of physiologic concentration ouabain on OK cell proliferation and to analyze its
mechanism.Methods:OK cells cultured under 0.2% Low Serum Culture Medium were treated with 1-30nM ouabain. Brdu incorporation
assay and MTT methods were used to detect OK cell proliferation; Western blot was used to determine the phosphorylation of Akt and
ERK of OK cells; LY294002, the PI3K/Akt specific inhibitor, and PD98059, the ERK1 /2 specific inhibitor, were used for interfering
PI3K/Akt and ERK1 /2 signaling pathway and observed their effect on ouabain induced OK cell proliferationResults:Low dose ouabain
(1 -30nM) stimulated OK cell growth. Ouabain could increase the phosphorylation of Akt and ERK in OK cells. The increase of OK cells
proliferation by ouabain was inhibited by PI3K inhibitor LY294002 or ERK1 /2 inhibitor PD98059.Conclusion:Low dose ouabain
promoted OK cell growth. PI3K/Akt and ERK1 /2 pathway have some effect on regulating this effect. |
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