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目的：采用抗核抗体谱(ANA 谱)十八项检测系统性红斑狼疮(SLE)患者，探讨其在SLE 诊治中的临床应用价值。方法：选择 2011 年2 月至2012 年12 月我院风湿科收治的370 例患者，其中系统性红斑狼疮(SLE)患者158 例、其他自身免疫性疾病患者 142例，其他疾病患者70 例，均采用间接免疫荧光法和免疫印迹法检测其ANA谱十八项，并对结果进行回顾性分析。结果：SLE 患者ANA 谱十八项中ANA (96.84%、55.47%、27.10%，P<0.01)、Anti-SSA/Ro60 (55.06%、14.34%、3.39%，P<0.01)、Anti- SSA/Ro52 (46.20%、12.83%、1.69%，P<0.01)、Anti-SmD1(31.65%、2.64%、1.69%，P<0.01)、Anti- U1-snRNP(25.95%、2.26%、0%，P<0.01)、AnuA (25.32%、0.75%、0%，P<0.01)、Anti- SSB/La (25.32%、5.66%、0.85%，P<0.01)、Anti-dsDNA (24.05%、0.38%、0%，P<0.01)、AHA (20.89%、0.38%、0%，P<0.01)、Anti-P0 (18.35%、0.38%、0%，P<0.01) 的阳性率均显著高于其他自身免疫性疾病患者和其他疾病患者。96.84%SLE 患者被检测出ANA阳性，以颗粒型为主(占51.63%)。81.65%的SLE 患者一次性检出2 项及以上抗体。结论：ANA 谱十八项检测有助于系统性红斑狼疮的筛查和诊断。

英文摘要:

Objective:To explore the clinical application value of detection of antinuclear antibodies (ANA) spectrum eighteen items in the diagnosis and treatment of systemic lupus erythematosus (SLE).Methods:Three hundred seventy inpatients admitted in the rheumatology department of our hospital fromFebruary 2011 to December 2012 were selected, including SLE patients (158 cases), other autoimmune diseases patients (142 cases) and other diseases patients (70 cases). The ANA spectrum eighteen items were detected with indirect immunoflurescence and immunoblotting methods. The results of ANA spectrum were retrospectively analyzed.Results:The positive rate of ten antibodies of ANA spectrum eighteen items showed significant defference between SLE group, other autoimmune diseases group and other disease group, including ANA (96.84%, 55.47%, 27.10%, P<0.01), Anti-SSA/Ro60 (55.06%, 14.34%, 3.39%, P<0.01), Anti-SSA/Ro52 (46.20%, 12.83%, 1.69%, P<0.01), Anti-SmD1 (31.65%, 2.64%, 1.69%, P<0.01), Anti- U1-snRNP (25.95%, 2.26%, 0%, P<0.01), AnuA(25.32%, 0.75%, 0%, P<0.01), Anti- SSB/La(25.32%, 5.66%, 0.85%, P<0.01), Anti-dsDNA(24.05%, 0.38%, 0%, P<0.01), AHA(20.89%, 0.38%, 0%, P<0.01), and Anti-P0(18.35%, 0.38%, 0%, P<0.01). The positive ANA were detected in 96.84% SLE patients and mainly with particle type (51.63%). Two or more antibodies were detected positive in 81.65%SLE patients at the same time.Conclusion:The detection of ANA spectrumeighteen items was valuable for the screening and diagosis of SLE.

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