| 牟颖 卢映蓉 蔡婷 吴飞.NGF电纺纤维的体外缓释研究[J].,2014,14(33):6404-6406 |
| NGF电纺纤维的体外缓释研究 |
| In Vitro Sustained Release Research of NGF Electrospun Fibers |
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| DOI： |
| 中文关键词: 静电纺丝纤维 神经导管 NGF PLLA 神经修复 |
| 英文关键词: Electrospun fibers Nerve conduit NGF PLLA Nerve repair |
| 基金项目:国家自然科学基金项目（81102406） |
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| 中文摘要: |
| 目的：研究担载神经生长因子（NGF）的静电纺丝纤维的表征，考察NGF电纺纤维对于周围神经修复的效果。方法：将NGF
水溶液分散于PLLA 溶液，通过W/O乳液法制备静电纺丝纤维，对纤维的形态、力学性能等进行表征，Elisa 方法测定NGF的体
外释放动力学，Alamer Blue 法检测试剂来考察纤维释放液对于PC12细胞增殖的影响。结果：NGF 电纺纤维具备良好的形态和
力学性质，直径为500-900 nm，纤维具备三维多孔结构。纤维的最大拉伸应力为2.50± 0.41 MPa。电纺纤维中NGF在体外可有效
释放9 天，累积释放量接近3000 pg。细胞活性实验结果显示，第1、3、5、7 天释放液的荧光强度与对照组相比有显著差异。结论：
担载NGF 的乳液法静电纺丝纤维有促进缺损周围神经修复的潜质。 |
| 英文摘要: |
| Objective:To investigate characterization and effect on peripheral nerve repair of NGF electrospun fibers.Methods:NGF solution was dispersed into PLLA solution, and then electrospun fibers were fabricated by W/O emulsion method. The morphology
and mechanical properties of the electrospun fibers were characterized and release kinetic of NGF in fibers was detected by NGF Elisa
kit. The effect of release solution on cell activity was tested by Alamer Blue.Results:The NGF emulsion electrospun fibers had good
morphology and mechanical properties. The fibers had porous structure with diameters between 500 nm and 900 nm. The maximum
tensile stress of fibers was 2.50± 0.41 MPa. NGF in fibers had sustained release for 9 days in vitro with a cumulative release of nearly
3000 pg. Statistical difference was found in fluorescence intensity of release solution compared to blank group in cell viability
experiment.Conclusion:NGF emulsion electrospun fibers provide a promising approach in the application of peripheral nerve repair. |
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