| 李琳 周倩倩 顾焕 李春岩 黄力.缺血性心脏病患者血清对血管内皮细胞增殖的影响[J].,2014,14(32):6242-6245 |
| 缺血性心脏病患者血清对血管内皮细胞增殖的影响 |
| The Effect of Serum of Patients with Coronary Heart Disease at Differentstages on the Proliferation of Human Umbilical Vein Endothelial Cells |
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| DOI： |
| 中文关键词: 缺血性心脏病 人脐静脉血管内皮细胞 血清 增殖 |
| 英文关键词: Ischemic heart disease HUVEC Serum Proliferation |
| 基金项目:国家自然科学基金面上项目(20775094) |
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| 中文摘要: |
| 目的：探讨不同阶段缺血性心脏病患者血清对人脐静脉血管内皮细胞(HUVEC)增殖的影响。方法：采集正常健康人群组(A
组)、缺血性心脏病高危人群组(B 组)、缺血性心脏病急性期患者组(C 组)、缺血性心脏病稳定期患者(D 组)的血清，分别作用于人
脐静脉血管内皮细胞不同时间，通过MTT 比色法，检测细胞的生长抑制率，观察其对HUVEC增殖的影响。结果：四组血清处理
HUVEC 24、48、72、96 小时后，B 组、C 组和D 组的细胞生长抑制率均显著低于A 组(P<0.05)，且C组和D 组24 小时的生长抑制
率显著低于B 组(P<0.05)，D组48、96小时显著低于B 组(P<0.05)，D 组96 小时显著低于C 组(P<0.05)。随着观察时间的延长，A、
B、C组患者血清的促增殖作用逐渐增强，48 h、72 h和96 h的生长抑制率均显著低于同组24h (P<0.05)；A组患者血清培养至96 h，
生长抑制率与同组48 h比较显著降低(P<0.05)。结论：从高危患者发展至缺血性心脏病，以及心血管疾病在度过急性期后，随着病
程和生存期的延长，其血清对细胞的促增殖作用逐渐增强。 |
| 英文摘要: |
| Objective:To observe the effect of different Periods serum on the proliferation of HUVEC.Methods:Acquisition in
normal healthy people group (group A), coronary heart disease, high-risk population groups (group B), a group of patients with acute
coronary heart disease (group C), coronary heart disease patients with plateau group (group D) of serum, respectively, to act on human
umbilical vein endothelial cells, cultivate to 24 h, 48 h, 72 hand 96 h to test absorbance value (OD value). MTT assay was used to
determine the cell growth inhibitory rate in vitro.Results:The growth inhibition ratios of group B, C, D were significantly lower than that
of groupA after being treated by different kinds of serum for 24, 48, 72 and 96 h (P<0.05), the growth inhibition ratios of group C and D
at 24 h were both significantly lower than that of group B at the same time points (P<0.05), the growth inhibition ratios of group D at 48,
96 h both significantly lower than that of group B at the same time points (P<0.05). With the extension of observation time, the
proliferation promotion effect of serum from group A, B, C enhanced gradually, the growth inhibition ratios at 48, 72, and 96 h were
significantly lower than that at 24 h of the same group (P<0.05), the growth inhibition ratios at 96 h of group A was significantly lower
than that at 48h of the same group(P<0.05).Conclusion:From the high-risk patients to ischemic heart disease, and cardiovascular disease
after acute period, with the extension of progression and survival, the proliferation promotion effect of serumgradually enhanced. |
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