| 冯娟 栗艳 孙阳 石小鹏 文爱东.大黄多糖抑制脂多糖引起的TLR-4/ NF-kB通路活化[J].,2014,14(31):6035-6038 |
| 大黄多糖抑制脂多糖引起的TLR-4/ NF-kB通路活化 |
| RheumTanguticumPolysaccharides Suppress Lipopolysaccharide InducedActivation of TLR-4/ NF-kB Pathway |
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| DOI： |
| 中文关键词: 大黄多糖 结肠癌 TLR-4 NF-kB |
| 英文关键词: Rheumtanguticumpolysaccharides Colorectal cancer TLR-4 NF-kB |
| 基金项目:国家自然科学基金项目（30973914） |
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| 中文摘要: |
| 目的：探讨唐古特大黄多糖(Rheum tanguticum Polysaccharid, RTP)对脂多糖(Lipopolysaccharide，LPS)刺激上调人结肠癌细
胞HT-29 中TLR-4/NF-kB通路活性的影响及其机制。方法：将HT-29 细胞分为对照组，LPS 处理组（1ug/mL，作用30 min, 1 h），RTP (1mg/mL, 提前LPS 30 min 给予)+LPS 处理组（1ug/mL，分别作用30 min, 1 h），采用免疫荧光法观察NF-kB 的细胞分布情况；Western Blot 法检测HT-29 细胞中IkB-alph, 磷酸化IkB-alpha的蛋白变化，以及细胞膜上TLR-4的水平。结果：RTP 可抑制LPS刺
激引起的HT-29细胞的NF-kB 核转位；可有效抑制I kB-alpha降解及IkB-alpha的磷酸化；可下调细胞膜上TLR-4 的表达。结论：RTP可能通过抑制LPS刺激引起的TLR-4 向细胞膜分布，从而抑制了NF-kB 信号通路的活化。 |
| 英文摘要: |
| Objective:To investigate the effect of rheum tanguticum polysaccharides (RTP) on the activity of TLR-4/NF-kB
pathway and the possible mechanisms in lipopolysaccharide (LPS) treated human colon cancer cells HT-29.Methods:HT-29 cells were
divided into control group, LPS-treated groups (1 滋g/mL, added for 30 min, and 1 h), RTP (1mg/mL, cells were pretreated with RTP for
30 min) and LPS-treated group (1 ug/mL, added for 30 min, and 1 h). Then, the distribution of NF-kB in cells was observed by
immunofluorescence; the expression of IkB-alpha, phosphorylated IkB-alpha, as well as TLR-4 in the cell membrane, was detected using western
blot.Results:RTP could inhibit LPS-stimulated nuclear translocation of NF-kB, degradation of IkB-alpha, phosphorylation of IkB-alpha, and
reduce TLR-4 expression in membrane.Conclusion:RTP may suppress the activation of NF-kB signaling pathway through inhibition of
LPS-stimulated TLR-4 distribution to cell membrane. |
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