| 王志远 张丽丽 顾金霞 李学奇.大鼠腹腔异位心脏移植模型建立及改进[J].,2014,14(31):6019-6021 |
| 大鼠腹腔异位心脏移植模型建立及改进 |
| The Establishment and Improvement of Heterotopic Heart TransplantationModel in Rats |
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| DOI： |
| 中文关键词: 大鼠 异位心脏移植 动物模型 Ono术式 |
| 英文关键词: Rat Heterotopic heart transplantation Animal models Ono surgical |
| 基金项目:国家自然科学基金项目（81102236/H1006）；黑龙江省博士后科研启动基金项目（LRB03-129） |
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| 中文摘要: |
| 目的：建立一种快速有效的大鼠腹腔异位心脏移植模型。方法：采用SD 大鼠作为受体，Wistar大鼠作为供体，行同种异位腹
腔心脏移植，术后给以CsA5 mg/kg/d 灌服，心脏移植手术方法采用改良的Ono 术式，观察改良的腹腔异位心脏移植各步骤所需
时间、术后成功率及主要并发症发生率。结果：共建立40 只大鼠异位心脏移植模型，手术成功率92.5 %。动脉吻合时间12.5± 2.3
min，静脉吻合时间12.3± 1.5 min，供心缺血时间37± 3.5 min，受体血管阻断时间34.2± 2.6 min，总手术时间90.2± 4.8 min，出现
的主要并发症为出血和供心复跳失败（各占5 %、2.5 %）。结论：改进的大鼠腹部异位心脏移植技术是一种简便、快速、有效、成功
率高的模型制作方法。 |
| 英文摘要: |
| Objective:To establish a rapid and effective heterotopic heart transplant model in rats.Methods:Allogeneic
abdominal heterotopic heat transplantation was performed to make the SD rats as recipients and Wistar rats as the donors. After surgery,
the rats were given the CsA5 mg/kg/d by gavage. The surgery of Ono style was further optimized based on the abdominal heterotopic
heart transplantation, the time for each step, success rate and main complications after surgery.Results:40 rats' heterotopic heart
transplantation models were established with the success rate of 92.5%, and the aortic anastomosis time was 12.5 ± 2.3 min, the
pulmonary artery anastomosis time was 12.3± 1.5 min, total donor heart ischemia time was 37± 3.5 min, vessel occlusion time was
34.2± 2.6 min, and total time surgery was 90.2± 4.8 min. The main complications were bleeding and/or resuscitation for heart failure
which occurred to 5%and 2.5%of experimental rats, respectively.Conclusion:Improved abdominal heterotopic heart transplantation is
a simple, rapid, effective and high success rate method of model establishment. |
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