| 吴林峰 雷小英 颜真 孙建斌 向安.肿瘤细胞对血清补体杀伤耐受的机制研究[J].,2014,14(30):5833-5836 |
| 肿瘤细胞对血清补体杀伤耐受的机制研究 |
| Exploring the Molecular Mechanismof Tumor Resistanceto Complement Attack of Human Serum |
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| DOI： |
| 中文关键词: 补体 补体调节蛋白 CD46 |
| 英文关键词: Complement Complement regulatory protein CD46 |
| 基金项目:国家自然科学基金青年基金项目（30901357） |
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| 摘要点击次数: 1099 |
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| 中文摘要: |
| 目的：探讨肿瘤细胞耐受血清补体杀伤的分子机制。方法：将表达不同血型抗原的肿瘤细胞与添加同血型血清的培养基孵
育，获得耐受血清杀伤的肿瘤细胞。无血清悬浮培养肿瘤干细胞，对耐受血清杀伤的肿瘤细胞和肿瘤干细胞分别提取mRNA，进
行反转录PCR，检测肿瘤干细胞干性标志物和补体调节蛋白的表达情况；干扰补体调节蛋白，检测肿瘤细胞对血清补体的杀伤情
况。结果：对分别表达血型抗原A、B 和H 的HT-29、KATO Ⅲ、MCF7 肿瘤细胞均获得耐受血清杀伤的耐受细胞，耐受细胞高表达
三种膜性补体调节蛋白CD46、CD55 和CD59，以及部分肿瘤干细胞干性标志物；spheroid 悬浮球肿瘤干细胞高表达上述三种膜
性补体调节蛋白，特别是CD46；在肿瘤细胞中腺病毒干扰CD46 的表达，可显著增强血清补体对肿瘤细胞的杀伤率。结论：补体
调节蛋白CD46 分子可通过增强肿瘤细胞的干性而介导肿瘤细胞对血清补体的杀伤耐受。 |
| 英文摘要: |
| Objective:The molecular mechanism of tolerance to complement of human serum in tumor cells was explored.Methods:Tumor cells resistant to the complement killing of human serum were obtained by culturing tumor cells expressing blood
antigen in 1640 medium with the addition of human serum and spheroid stem-like tumor cells were cultured in medium without serum.
mRNA was extracted from resistant tumor cells and spheroid cells and reverse-transcript PCR was performed to detect the expression of
markers and complement regulatory proteins; the cytolysis rate was determined by interfering CD46 in tumor cells with recombinant
adenovirus.Results:Resistant HT-29, KATO Ⅲ and MCF7 cells expressing blood antigen A, B and H, respectively to complement
attack of human serumexpressed higher level of membrane complement regulatory proteins CD46, CD55 and CD59 than that of parental
tumor cells. In addition, spheroid stem-like tumor cells displayed a high level of CD46, CD55 and CD59, especially CD46. Finally,
down-regulation of CD46 expression with adenovirus can significantly enhance the cytolysis of tumor cells induced by human serum.Conclusion:Complement regulatory molecular CD46 can mediate tumor resistance to complement killing by up-regulating the stemness
of tumor cells. |
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