| 肖慧 谷文萍 胡珏 王振.Ephrin-B2 促进大鼠局灶脑缺血再灌注后VEGF表达及血管新生[J].,2014,14(30):5824-5828 |
| Ephrin-B2 促进大鼠局灶脑缺血再灌注后VEGF表达及血管新生 |
| Ephrin-B2 Enhances Expression of VEGF and Angiogenesisafter Focal Cerebral Ischemia in Rats |
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| DOI： |
| 中文关键词: 脑缺血 Ephrin-B2 血管新生 VEGF |
| 英文关键词: Cerebral ischemia Ephrin-B2 Angiogenesis VEGF |
| 基金项目:湖南省科技厅科技计划项目(2012FJ6031) |
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| 中文摘要: |
| 目的：探讨Ephrin-B2对大鼠脑缺血再灌注后脑组织中血管新生的调节作用及其可能的机制。方法：雄性SD 大鼠随机分为
正常组及、缺血再灌注组及Ephrin-B2 干预组，后两组再分为4天、7天、14 天、28 天亚组；线栓法制备局灶性大脑中动脉缺血再
灌注模型；改良神经功能评分(modified neurological severity scores mNSS) 评分法对各时间点模型进行评分；Western blot 及荧光
定量PCR 检测缺血脑组织中血管内皮生长因子（Vascular Endothelial Growth Factor VEGF）的表达；以免疫荧光双标法定位VEGF
表达的细胞类型；以CD31+BrdU计数缺血半暗带中新生微血管密度(microvessel density MVD)。结果：Ephrin-B2 干预组与缺血再
灌注组各时间点亚组比较，新生微血管密度测定计数较缺血再灌注组均显著增加（P<0.05），神经功能评分均显著降低（P<0.05），
VEGF mRNA 水平及蛋白表达水平均显著增加（P<0.05），VEGF主要表达于CD31 阳性的血管内皮细胞。结论：Ephrin-B2 通过上
调VEGF的表达促进脑缺血再灌注后缺血半暗带血管新生，从而促进神经功能缺失的修复。 |
| 英文摘要: |
| Objective:To explore the effect of Ephrin-B2 on angiogenesis in the ischemic penumbra after transient focal cerebral
ischemia in rats; To clarify the mechanism of Ephrin-B2 triggering angiogenesis after transient focal cerebral ischemia.Methods:Sprague-Dawley rats were randomly divided into three groups: normal group, ischemic-reperfusion group and Ephrin-B2 treated group,
the last two groups were randomly divided into isubgroups of ischemic -reperfusion on the 4th, 7th, 14th, and 28th day. Longa
methods were used to make the focal middle cerebral artery occlusion model, and the ischemic brain was reperfused 2 hours after
occlusion; Ephrin-B2 protein was administered intracerebroventricularly to examine its effect on angiogenesis and behavioral recovery.
Microvessel density (MVD)was quantifiedbycountingthe number ofCD31+andBrdU+cells. Ratswere subjected to neurologic functional
tests by modified neurological severity scores (mNSS) before sacrificed. Western-blot and quantitative real-time reverse-transcription
Polymerase chain reaction were used to detect the dynamic expression profile of VEGF（Vascular Endothelial Growth Factor) in the
penumbra cortex. Double immunoflurence was used to speculate the location and the co-expression of VEGF with blood vessels.Results:Compared to the normal group, mNSS of Ephrin-B2 treated group gradually declined (P＜0.05), the MVD of penumbra cortex in
Ephrin-B2 treated group increased(P＜0.05), and VEGF protein and mRNA level of the penumbra cortex in Ephrin-B2 treated group was
highly upregulated after reperfusion in all of subgroups (P＜0.05); Double immunoflurence indicated that VEGF were expressed in blood
vessels.Conclusion:Ephrin-B2 may improve post-stroke functional recovery by enhancing VEGF-induced angiogenesis. |
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