| 邱艳芬 王梦溪 马肃 张睿 刘志杰.纤粘连蛋白在实验性牙周炎鼠牙龈组织中的表达[J].,2014,14(30):5821-5823 |
| 纤粘连蛋白在实验性牙周炎鼠牙龈组织中的表达 |
| Expression of Fibronectin in Gingival Tissues of Experimental Periodontitis |
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| DOI： |
| 中文关键词: 纤粘连蛋白 牙周炎 组织形态 免疫组化 |
| 英文关键词: Fibronectin Periodontitis Tissue morphous Immunohistochemistry |
| 基金项目:黑龙江省科技厅基金项目（D2004-43） |
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| 中文摘要: |
| 目的：观察分析大鼠实验性牙周炎牙龈组织中纤粘连蛋白(fibronectin，FN)的表达及意义。方法：26 只8 周龄雄性Wistar大
鼠，随机分为局部丝线结扎高糖软食4 周和8 周两组，每组实验动物10只，空白对照组3只。运用免疫组化方法，观察分析FN 在
健康牙龈组织中、牙周炎牙龈组织中的表达及意义。结果：健康牙龈组织中，FN相对均匀弥漫表达于整个牙龈结缔组织基质内；
牙周炎牙龈组织中，FN 表达具有部位特异性，即上皮下结缔组织最上部基质内FN 表达明显下调；结合上皮根端基底细胞基底面
下FN 表达明显上调，表达强度和范围随结合上皮根向增生程度的增加而增强。结论：炎性刺激下调炎症中心区牙龈结缔组织基
质内FN 表达；炎症刺激上调结合上皮根端基底细胞基底面下FN表达，其表达强度和范围随结合上皮根向增生程度的增加而增
强扩大，暗示FN 参与牙龈结合上皮根向增生迁移活动。 |
| 英文摘要: |
| Objective:To observe the expression of fibronectin in gingival tissues of experimental periodontitis.Methods:A total
of 26 Wistar rats, male, eight-week-old, were used, 20 of which were randomly chosen as experimental objects and 6 as control ones. The
experimental objects were randomly divided into 2 groups with equal numbers, then ligated with silk suture and fed with high glucose
diet for 4 weeks and 8 weeks respectively. By using immunohistochemistry, the expression of fibronectin healthy gingival tissues and
periodontitis gingival tissues was observed.Results:In healthy gingival tissues, fibronectin showed a relative diffuse distribution in the
gingival connective tissue matrix. In periodontitis gingival tissues, the expression of fibronectin indicated a regional specificity.
Fibronectin expressed decreasingly in the upmost subepithelium gingival connective tissue matrix, while increasely in the basal surface of
base cell beneath the junctional epithelium root apex. The expression intensity and circumscription was enhanced with junctional
epithelium root apex proliferation.Conclusion:Inflammation stimulus decreased the expression of fibronectin in the centrinflammation's
gingival connective tissue matrix, and breakdown collagen-matrix wire frame texture; Inflammation stimulus increased
the expression of fibronectin in the basal surface of base cell of the junctional epithelium root apex. The expression intensity and
circumscription were enhanced with junctional epithelium root apex proliferation; Fibronectin possibly participates in epithelium cells
proliferationand transference of junctional epitheliumfor root apex direction. |
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