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目的：探讨羟乙基淀粉沉淀（HES）法分离脐血单个核细胞（MNCs)的效果。方法：采集脐血31 份，应用羟乙基淀粉（HES）沉淀飞和密度离心（Ficoll)法分离脐血MNCs，、纯化CD3+细胞、CD14+细胞、CD34+细胞，以台盼蓝拒染法检测细胞活力。结果：HES 法MNCs 回收率、CFU-GM 计数高于Ficoll 法（85.29 ± 3.79 VS 47.06 ± 4.61，t=35.67，P<0.05；67.31 ± 11.57/l× 105 MNCs VS 28.54± 6.39/l× 105MNCs，t=16.33，P<0.05）；HES 法分离的MNCs 中CD3+、CD14+、CD34+细胞数均高于Ficoll 法（t=5.76，t=2.51， t=6.67，P<0.05)。结论：HES 法分离MNCs可获得较好的回收率，是人脐血干细胞理想的分离方法。

英文摘要:

Objective:To investigate the separation effect of hydroxyethyl starch (HES) precipitation for nucleated cells(MNCs).Methods:31 copies of cord blood were collected and separated by HES separation and density centrifugation (Ficoll) for MNCs, the CD3+ cells, CD14+ cells, and CD34+ cells, purified by immunomagnetic technique, and cell viability were detected by trypan blue staining.Results:The rate of recollection for MNCs and CFU-GM counts using HES method were higher than that using of Ficoll method (85.29± 3.79 VS 47.06± 4.61, t=35.67, P<0.05; 67.31± 11.57/l× 105MNCs VS 28.54± 6.39/l× 105MNCs, t=16.33, P<0.05); CD3+, CD14+, CD34+ cells, using HES method were higher than that of Ficoll method (t=5.76, t=2.51, t=6.67, P<0.05).Conclusion:HES method is an appropriate method for separating HSCs.

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