| 卢映蓉 牟颖 李丽云 蔡婷 吴飞.血管内皮生长因子(VEGF)乳液法电纺纤维膜的体外研究[J].,2014,14(28):5443-5445 |
| 血管内皮生长因子(VEGF)乳液法电纺纤维膜的体外研究 |
| Study on the Vascular Endothelial Growth Factor (VEGF) emulsionElectrospun fiber Membrane in Vitro |
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| DOI： |
| 中文关键词: 血管内皮生长因子 乳液法 电纺纤维膜 PLGA 血管再生 |
| 英文关键词: VEGF Emulsion method Electrospun fiber membrane PLGA Revascularization |
| 基金项目:国家自然科学基金项目(81102406) |
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| 中文摘要: |
| 目的：研究担载血管内皮生长因子(VEGF)的乳液法电纺纤维膜的亲水性能、外观形态和机械性能，纤维膜中VEGF 的包封
率和体外释放动力学，为评价其能否应用于血管再生领域的研究奠定基础。方法：将VEGF 水溶液通过W/O 乳液法制备成缓释
VEGF的生物可降解的丙交酯-乙交酯共聚物(PLGA)静电纺丝纤维膜，对该纤维膜的接触角、外观形态、机械性能进行表征，Elisa
法测定该纤维膜的体外14 天的释放行为，分别观察纤维膜释放0 天、7 天、14 天后的电镜图。结果：加入VEGF后，纤维膜的接触
角由140.0° 减小到136.1° ，亲水性增强，具有类似细胞外基质(ECMs)网状结构和良好的力学性能，纤维膜第1 天的突释不超过
载药量的50%，电镜图下显示纤维膜释放1 周时纤维发生断裂。结论：通过乳液法制备的担载VEGF的电纺纤维膜具有良好的物
理性能，能够持续缓释VEGF，可作为血管再生的组织工程支架进行深入研究。 |
| 英文摘要: |
| Objective:To investigate the hydrophilic performance, morphology and mechanical properties of emulsion electrospun
fiber membrane loading vascular endothelial growth factor (VEGF), the coating rate and release kinetics of VEGF in vitro in the fiber
membrane, and evaluate whether it could be applied in the research of angiogenesis.Methods:VEGF aqueous solution was prepared into
a VEGF sustained-release PLGA electrospun fiber membrane by W/O emulsion method. The contact Angle, exterior morphology, and
mechanical properties of the fiber membrane were characterized. Elisa method was to detect the release behavior of the fiber membrane
in vitro. The fiber membrane SEM0 day, 7 days, and 14 days after release were observed respectively.Results:After adding VEGF, the
contact Angle of fiber membrane reduced from 140.0° to 136.1° , its hydrophilicity enhanced, the fiber membrane had similar
extracellular matrix (ECMs) mesh structure and good mechanical properties, initial burst release fiber membrane was no more than 50%
drug loading capacity on the first day, fiber membrane had a break under SEMin a week.Conclusion:VEGF emulsion electrospun fiber
membrane had good physical properties, continuous sustained release of VEGF, which could be used as angiogenesis tissue engineering
scaffolds for further research. |
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