| 张春晓 袁杰 叶珑伟 李天侠 宋纯.新城疫病毒（NDV）D90 抑制口腔鳞癌细胞系迁移和侵袭的研究[J].,2014,14(28):5436-5439 |
| 新城疫病毒（NDV）D90 抑制口腔鳞癌细胞系迁移和侵袭的研究 |
| Study on Newcastle Disease Virus(NDV) D90 Inhibited the Migrationand Invasion of Oral Squamous Cell Carcinoma Cell Lines |
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| DOI： |
| 中文关键词: 新城疫病毒D90 口腔鳞状细胞癌细胞 能动性 迁移和侵袭 |
| 英文关键词: NDV D90 OSCC cells Motility Migration and invasion |
| 基金项目:黑龙江省自然科学基金项目（D200972） |
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| 中文摘要: |
| 目的：证明NDV D90 是否具有抑制口腔鳞癌细胞系迁移和侵袭的能力。方法：免疫荧光染色法检测D90对细胞微管和微丝
形态的改变；Transwell 法检测D90 对HN-6 细胞迁移和侵袭率的抑制作用；蛋白印迹法检测D90 对于SP1、RECK、MMP-2 和
MMP-9 表达的影响；明胶酶谱法用于检测MMP-2 和MMP-9 活性的改变。结果：实验结果表明，D90 通过改变细胞的微管和微丝
形态来抑制细胞的能动性；D90 具有抑制HN-6 细胞的迁移和侵袭的功能。同时，D90 通过下调SP1 和上调RECK的表达来抑制
MMP-2 和MMP-9 的表达和活性。结论：NDV D90 能够有效地抑制口腔鳞癌细胞系HN-6 的迁移和侵袭，为一种新的抗肿瘤制剂
提供了临床试验基础。 |
| 英文摘要: |
| Objective:To detect that whether NDV D90 has the ability to inhibit the migration and invasion of oralsquamous cell
carcinoma cell line.Methods:Immunocyochemistry was used to detect the changes of microtubules and microfilaments induced by D90;
the effect of D90 migration and invasion rate was assayed by Transwell assay; the change of SP1, RECK, MMP-2 and -9 expression
induced by D90 was detected by western blot and the change of MMP-2 and -9 activity was detected by gelatin zymography.Results:The
results demonstrated that D90 changed the morphology of microtubules and microfilaments to inhibit the cell motility; D90 possessed the
ability of inhibiting the migration and invasion rate of HN-6 cells. Meanwhile, D90 significantly decreased the expression of sp1 and
increased the expression of RECK to suppress the expression and activity of MMP-2 and MMP-9.Conclusion:NDV D90 possessed the
ability to inhibit obviously the migration and invasion of HN-6 cell.The finding provided a basis for clinical trials of NDV D90 to be a
new antitumor agents. |
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