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目的：将传统的外周血单个核细胞分离计数方法加以改进，并探讨2 型糖尿病（DM）患者外周血单个核细胞数量与疾病的相关性。方法：密度梯度离心分离30 例健康体检者单个核细胞，不同染色稀释液重悬细胞，充入改良牛鲍计数板连续计数5 天，计算批内、批间变异；采用上述方法分离2 型DM患者抗凝全血29 例，计算单个核细胞数量；采用全自动生化分析仪检测对照组和 2 型DM组患者血脂。结果：两组的单个核细胞经3 种前处理染液稀释重悬细胞后，计数结果间无统计学差异(P＞0.05)；2 型DM 组与健康对照组比较，外周血单个核细胞计数绝对值无显著性差异(P＞0.05)，血清TG、TC、LDL水平明显升高，差别具有统计学意义（P＜0.05），而其他指标结果间比较无差异。在对照组中，单个核细胞计数结果与白细胞呈正相关（r=0.611,P＜0.05），与血清 TC、LDL水平呈负相关（r1=-0.550, r2=-0.605，P＜0.05）。而T2DM组，单个核细胞计数结果仅与LDL水平呈负相关（r=-0.104, P＜ 0.05）。结论：外周血单个核细胞数与2 型糖尿病无明显相关性。

英文摘要:

Objective:To improve traditional counting method of peripheral blood mononuclear cells, and investigate association between quantity of peripheral blood mononuclear cell and type 2 diabetes mellitus (DM).Methods:Mononuclear cells of 30 healthy people were obtained by density gradient centrifugation, mixing with different colored dilution and counting in continuous 5 days, establishing interassay variation and intraassay coefficient of variation;Peripheral blood mononuclear cell of 29 type 2 diabetic patients were gained by above-mentioned method; Plasma lipids were measured by 7600-020E automatic biochemistry analyzer.Results:Mononuclear cells of healthy people were mixed by staining solutions, there is no statistics significance among counted numbers (P＞ 0.05); Comparing with healthy control, there is no statistics significance between absolute value of Mononuclear cells of type 2 diabetic patients (P＞0.05), serumTG, TC, LDL were higher obviously, with statistics significance（P＜0.05), there is difference among results of other index. In control, counted numbers of mononuclear cells were positive correlation with leucocyte (r=0.611,P＜0.05）, negative correlation with TC, LDL (r1=-0.550, r2=-0.605, P＜0.05). In type 2 diabetic group, counted numbers of mononuclear cells was only negative correlation with LDL (r=-0.104, P＜0.05).Conclusion:Numbers of mononuclear cells is no obviously association with type 2 diabetic patients.

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